The separation of dimethylarsinic acid, methylarsonous acid, methylarsonic acid, arsenate and dimethylarsinous acid on the hamilton PRP-X100 anion-exchange column

Jürgen Gailer, Sean Madden, William R. Cullen, M. Bonner Denton

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

In order to separate the potential arsenite metabolites methylarsonous acid and dimethylarsinous acid from arsenite, arsenate, methylarsonic acid and dimethylarsinic acid, the pH-dependent retention behaviour of all six arsenic compounds was studied on a Hamilton PRP-X100 anion-exchange column with 30 mM phosphate buffers (pH 5, 6, 7, 8 and 9) containing 20% (v/v) methanol as mobile phase and employing an inductively coupled plasma atomic emission spectrometer (ICP-AES) as the arsenic-specific detector. Baseline separation of dimethylarsinic acid, methylarsonous acid, methylarsonic acid, arsenate and dimethylarsinous acid was achieved with a 30 mmol dm-3 phosphate buffer (pH 5)-methanol mixture (80:20, v/v) in 25 min. Arsenite is not baseline-separated from dimethylarsinic acid under these conditions.

Original languageEnglish (US)
Pages (from-to)837-843
Number of pages7
JournalApplied Organometallic Chemistry
Volume13
Issue number11
DOIs
StatePublished - 1999

Keywords

  • Arsenate
  • Arsenite
  • Dimethylarsinic acid
  • Dimethylarsinous acid
  • HPLC-ICP-AES
  • Methylarsonic acid
  • Methylarsonous acid
  • Speciation

ASJC Scopus subject areas

  • Chemistry(all)
  • Inorganic Chemistry

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