The separation of peptide mormone diastereoisomers by reverse phase high pressure liquid chromatography. Factors affecting separation of oxytocin and its diastereoisomers-structural implications.

B. Larsen, B. L. Fox, M. Burke, Victor J Hruby

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Abstract

Experimental conditions and parameters involved in high performance liquid chromatography (HPLC) separations of the peptide hormone oxytocin and seven of its diastereoisomers, namely [1-hemi-D-cystine]-, (2,D-tyrosine]-, [4-D-glutamine]-, [5-D-asparagine]-, [6-hemi-D-cystine-], [7-D-proline]-, [8-D-leucine]-oxytocin, on reverse phase columns were investigated. The effects of solvent, pH, and salt concentration were studied. Using the solvent systems 10% tetrahydrofuran-ammonium acetate buffer or 18% acetonitrile-ammonium acetate buffer and the μBondapak C18 support, oxytocin was separated from each of its diastereoisomers under all conditions studied, but the order of elution of diastereoisomers was highly dependent on solvent and to a lesser extent on pH. Separations of the hormone and its diastereoisomers on reverse phase HPLC and on classical partition chromatography on Sephadex G-25 were compared. The results are discussd in terms of the interactions of the solute with the reverse phase column and the solvent system. Implications of these findings in terms of the different solution conformations of the peptides are discussed.

Original languageEnglish (US)
Pages (from-to)12-21
Number of pages10
JournalInternational Journal of Peptide and Protein Research
Volume13
Issue number1
StatePublished - 1979

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High pressure liquid chromatography
Oxytocin
Reverse-Phase Chromatography
High Pressure Liquid Chromatography
Peptides
Cystine
High performance liquid chromatography
Buffers
Peptide Hormones
Asparagine
Chromatography
Glutamine
Proline
Leucine
Tyrosine
Conformations
Salts
Hormones
ammonium acetate

ASJC Scopus subject areas

  • Biochemistry

Cite this

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title = "The separation of peptide mormone diastereoisomers by reverse phase high pressure liquid chromatography. Factors affecting separation of oxytocin and its diastereoisomers-structural implications.",
abstract = "Experimental conditions and parameters involved in high performance liquid chromatography (HPLC) separations of the peptide hormone oxytocin and seven of its diastereoisomers, namely [1-hemi-D-cystine]-, (2,D-tyrosine]-, [4-D-glutamine]-, [5-D-asparagine]-, [6-hemi-D-cystine-], [7-D-proline]-, [8-D-leucine]-oxytocin, on reverse phase columns were investigated. The effects of solvent, pH, and salt concentration were studied. Using the solvent systems 10{\%} tetrahydrofuran-ammonium acetate buffer or 18{\%} acetonitrile-ammonium acetate buffer and the μBondapak C18 support, oxytocin was separated from each of its diastereoisomers under all conditions studied, but the order of elution of diastereoisomers was highly dependent on solvent and to a lesser extent on pH. Separations of the hormone and its diastereoisomers on reverse phase HPLC and on classical partition chromatography on Sephadex G-25 were compared. The results are discussd in terms of the interactions of the solute with the reverse phase column and the solvent system. Implications of these findings in terms of the different solution conformations of the peptides are discussed.",
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AU - Burke, M.

AU - Hruby, Victor J

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