The Suppressor of AAC2 Lethality SAL1 Modulates Sensitivity of Heterologously Expressed Artemia ADP/ATP Carrier to Bongkrekate in Yeast

Monika Wysocka-Kapcinska, Beata Torocsik, Lilla Turiak, George Tsaprailis, Cynthia L. David, Andrea M. Hunt, Karoly Vekey, Vera Adam-Vizi, Roza Kucharczyk, Christos Chinopoulos

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The ADP/ATP carrier protein (AAC) expressed in Artemia franciscana is refractory to bongkrekate. We generated two strains of Saccharomyces cerevisiae where AAC1 and AAC3 were inactivated and the AAC2 isoform was replaced with Artemia AAC containing a hemagglutinin tag (ArAAC-HA). In one of the strains the suppressor of ΔAAC2 lethality, SAL1, was also inactivated but a plasmid coding for yeast AAC2 was included, because the ArAACΔsal1Δ strain was lethal. In both strains ArAAC-HA was expressed and correctly localized to the mitochondria. Peptide sequencing of ArAAC expressed in Artemia and that expressed in the modified yeasts revealed identical amino acid sequences. The isolated mitochondria from both modified strains developed 85% of the membrane potential attained by mitochondria of control strains, and addition of ADP yielded bongkrekate-sensitive depolarizations implying acquired sensitivity of ArAAC-mediated adenine nucleotide exchange to this poison, independent from SAL1. However, growth of ArAAC-expressing yeasts in glycerol-containing media was arrested by bongkrekate only in the presence of SAL1. We conclude that the mitochondrial environment of yeasts relying on respiratory growth conferred sensitivity of ArAAC to bongkrekate in a SAL1-dependent manner.

Original languageEnglish (US)
Article numbere74187
JournalPLoS One
Volume8
Issue number9
DOIs
StatePublished - Sep 20 2013

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Bongkrekic Acid
ATP Translocases Mitochondrial ADP
Artemia
Yeast
Yeasts
Mitochondria
yeasts
Hemagglutinins
Carrier Proteins
mitochondria
transport proteins
hemagglutinins
Dolphins
Adenine Nucleotides
Poisons
Strain control
Growth
Membrane Potentials
Glycerol
Adenosine Diphosphate

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The Suppressor of AAC2 Lethality SAL1 Modulates Sensitivity of Heterologously Expressed Artemia ADP/ATP Carrier to Bongkrekate in Yeast. / Wysocka-Kapcinska, Monika; Torocsik, Beata; Turiak, Lilla; Tsaprailis, George; David, Cynthia L.; Hunt, Andrea M.; Vekey, Karoly; Adam-Vizi, Vera; Kucharczyk, Roza; Chinopoulos, Christos.

In: PLoS One, Vol. 8, No. 9, e74187, 20.09.2013.

Research output: Contribution to journalArticle

Wysocka-Kapcinska, M, Torocsik, B, Turiak, L, Tsaprailis, G, David, CL, Hunt, AM, Vekey, K, Adam-Vizi, V, Kucharczyk, R & Chinopoulos, C 2013, 'The Suppressor of AAC2 Lethality SAL1 Modulates Sensitivity of Heterologously Expressed Artemia ADP/ATP Carrier to Bongkrekate in Yeast', PLoS One, vol. 8, no. 9, e74187. https://doi.org/10.1371/journal.pone.0074187
Wysocka-Kapcinska, Monika ; Torocsik, Beata ; Turiak, Lilla ; Tsaprailis, George ; David, Cynthia L. ; Hunt, Andrea M. ; Vekey, Karoly ; Adam-Vizi, Vera ; Kucharczyk, Roza ; Chinopoulos, Christos. / The Suppressor of AAC2 Lethality SAL1 Modulates Sensitivity of Heterologously Expressed Artemia ADP/ATP Carrier to Bongkrekate in Yeast. In: PLoS One. 2013 ; Vol. 8, No. 9.
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