The T-cell-independent immune response to the hapten NP uses a large repertoire of heavy chain genes

Nancy Maizels, Alfred Bothwell

Research output: Contribution to journalArticlepeer-review

99 Scopus citations

Abstract

Hybridomas generated from C57BL/6 mice immunized with the hapten NP coupled to ficoll, a T-cell-independent carrier, produce monoclonal antibodies that use a large repertoire of VH regions and light chains. This contrasts with the homogeneity of the strain-specific response to NP observed with T-cell-dependent carriers, where most of the antibodies use a single VH region, V186.2, in combination with the lambda-1 light chain. There is no evidence for somatic mutation in any of the sequenced regions of the antibodies generated by NP-ficoll. Thus T cell participation is required for the homogeneity of the strain-specific hapten response, and probably for somatic mutation as well.

Original languageEnglish (US)
Pages (from-to)715-720
Number of pages6
JournalCell
Volume43
Issue number3 PART 2
DOIs
StatePublished - Dec 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint

Dive into the research topics of 'The T-cell-independent immune response to the hapten NP uses a large repertoire of heavy chain genes'. Together they form a unique fingerprint.

Cite this