The tannin theory of methanogenic toxicity

James A Field, S. Kortekaas, G. Lettinga

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The tannin theory describes the effectiveness of tannic compounds as bacterial inhibitors based on their molecular weight (MW). The inhibition is postulated to result from the hydrogen-bonding of the tannins with bacterial proteins (i.e. enzymes). Oligomeric tannins are expected to be the most effective inhibitors. The oligomeric tannins form stronger hydrogen-bonds with proteins than do their monomers. The high-MW tannins are also reactive with proteins in the bulk solution; however, they are not able to penetrate to bacterial proteins. In this study, tannins were polymerized by autoxidation to colored compounds in order to investigate the role of tannin MW on methanogenic toxicity. The autoxidation of catechin, a tannin monomer, paralleled the expected toxicity as predicted by the tannin theory. The toxicity increased as it was polymerized to oligomeric tannins and decreased as the oligomers were converted to non-toxic high-MW tannins. The autoxidation of green-tea tannins, oligomeric tannins, did not lead to extensive polymerization reactions and no accumulation of high MW tannins was observed. Instead, the toxic green-tea tannins were transformed to colored non-tannic compounds of variable MW, which were non-toxic.

Original languageEnglish (US)
Pages (from-to)241-262
Number of pages22
JournalBiological Wastes
Volume29
Issue number4
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Tannins
tannin
Toxicity
toxicity
Molecular weight
protein
Bacterial Proteins
tea
inhibitor
Hydrogen bonds
Monomers
hydrogen
Proteins
Catechin
Poisons

ASJC Scopus subject areas

  • Pollution
  • Waste Management and Disposal

Cite this

The tannin theory of methanogenic toxicity. / Field, James A; Kortekaas, S.; Lettinga, G.

In: Biological Wastes, Vol. 29, No. 4, 1989, p. 241-262.

Research output: Contribution to journalArticle

Field, James A ; Kortekaas, S. ; Lettinga, G. / The tannin theory of methanogenic toxicity. In: Biological Wastes. 1989 ; Vol. 29, No. 4. pp. 241-262.
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