The tolerance of lignin peroxidase and manganese-dependent peroxidase to miscible solvents and the in vitro oxidation of anthracene in solvent: Water mixtures

James A Field, Ronald H. Vledder, John G. Van Zelst, Wim H. Rulkens

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Lignin peroxidase (LiP) and manganese-dependent peroxidase (MnP) titers in extracellular fluids of five-day-old Bjerkandera sp. strain BOS55 cultures and semipurified preparations from Phanerochaete chrysosporium were evaluated for their tolerance to miscible solvents. MnP assayed with 2,6-dimethoxyphenol (DMP) was found to retain 20% of its normal activity in the presence of 45-58% v/v acetone, 31-34% v/v ethanol, and 29% 2-propanol. LiP assayed with veratryl alcohol (VA) was about 2-3 times less tolerant to solvents compared to MnP. Methanol and 2-methoxyethanol were extremely inhibitory to LiP. The subtoxic concentrations of acetone were sufficient to remarkably increase the solubility of the model apolar pollutant, anthracene. Each 10% increment in the solvent concentration could be correlated to a 6.4-fold increase in the soluble anthracene concentration. When the peroxidases were incubated in vitro with anthracene as a substrate, acetone was essential for the elimination of anthracene and concomitant formation of anthraquinone. We observed for the first time a H2O2-enhanced oxidation of a polycyclic aromatic hydrocarbon, anthracene, by MnP when enough acetone was used (40% v/v). At acetone concentrations ranging from 5-60%, MnP was also able to oxidize anthracene in the absence of exogenous H2O2 additions but to a lesser extent. The optimum acetone concentration for the LiP-mediated oxidation of anthracene was 10% v/v. The in vitro oxidation rates with semipurified LiP (65 nmol VA min-1 ml-1) and MnP (33 nmol DMP min-1 ml-1) at the optimal solvent concentration was 4.3 and 4.0 mg anthracene l-1 d-1, respectively. The rate of anthracene conversion by LiP was similar to the anthracene dissolution rate in 10% acetone. In contrast, the conversion by MnP was estimated to be several orders of magnitude slower than the dissolution kinetics in 40% acetone.

Original languageEnglish (US)
Pages (from-to)300-308
Number of pages9
JournalEnzyme and Microbial Technology
Volume18
Issue number4
DOIs
StatePublished - Mar 1996
Externally publishedYes

Fingerprint

manganese peroxidase
Anthracene
Lignin
Manganese
Acetone
Oxidation
Water
Dissolution
Alcohols
Coriolaceae
anthracene
lignin peroxidase
In Vitro Techniques
Phanerochaete
Peroxidases
Anthraquinones
2-Propanol

Keywords

  • Cosolvent
  • Dissolution rate
  • Lignin peroxidase
  • Manganese-dependent peroxidase
  • Miscible solvent
  • Polycyclic aromatic hydrocarbon
  • Solubility

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

The tolerance of lignin peroxidase and manganese-dependent peroxidase to miscible solvents and the in vitro oxidation of anthracene in solvent : Water mixtures. / Field, James A; Vledder, Ronald H.; Van Zelst, John G.; Rulkens, Wim H.

In: Enzyme and Microbial Technology, Vol. 18, No. 4, 03.1996, p. 300-308.

Research output: Contribution to journalArticle

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abstract = "Lignin peroxidase (LiP) and manganese-dependent peroxidase (MnP) titers in extracellular fluids of five-day-old Bjerkandera sp. strain BOS55 cultures and semipurified preparations from Phanerochaete chrysosporium were evaluated for their tolerance to miscible solvents. MnP assayed with 2,6-dimethoxyphenol (DMP) was found to retain 20{\%} of its normal activity in the presence of 45-58{\%} v/v acetone, 31-34{\%} v/v ethanol, and 29{\%} 2-propanol. LiP assayed with veratryl alcohol (VA) was about 2-3 times less tolerant to solvents compared to MnP. Methanol and 2-methoxyethanol were extremely inhibitory to LiP. The subtoxic concentrations of acetone were sufficient to remarkably increase the solubility of the model apolar pollutant, anthracene. Each 10{\%} increment in the solvent concentration could be correlated to a 6.4-fold increase in the soluble anthracene concentration. When the peroxidases were incubated in vitro with anthracene as a substrate, acetone was essential for the elimination of anthracene and concomitant formation of anthraquinone. We observed for the first time a H2O2-enhanced oxidation of a polycyclic aromatic hydrocarbon, anthracene, by MnP when enough acetone was used (40{\%} v/v). At acetone concentrations ranging from 5-60{\%}, MnP was also able to oxidize anthracene in the absence of exogenous H2O2 additions but to a lesser extent. The optimum acetone concentration for the LiP-mediated oxidation of anthracene was 10{\%} v/v. The in vitro oxidation rates with semipurified LiP (65 nmol VA min-1 ml-1) and MnP (33 nmol DMP min-1 ml-1) at the optimal solvent concentration was 4.3 and 4.0 mg anthracene l-1 d-1, respectively. The rate of anthracene conversion by LiP was similar to the anthracene dissolution rate in 10{\%} acetone. In contrast, the conversion by MnP was estimated to be several orders of magnitude slower than the dissolution kinetics in 40{\%} acetone.",
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T1 - The tolerance of lignin peroxidase and manganese-dependent peroxidase to miscible solvents and the in vitro oxidation of anthracene in solvent

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AU - Field, James A

AU - Vledder, Ronald H.

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AU - Rulkens, Wim H.

PY - 1996/3

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N2 - Lignin peroxidase (LiP) and manganese-dependent peroxidase (MnP) titers in extracellular fluids of five-day-old Bjerkandera sp. strain BOS55 cultures and semipurified preparations from Phanerochaete chrysosporium were evaluated for their tolerance to miscible solvents. MnP assayed with 2,6-dimethoxyphenol (DMP) was found to retain 20% of its normal activity in the presence of 45-58% v/v acetone, 31-34% v/v ethanol, and 29% 2-propanol. LiP assayed with veratryl alcohol (VA) was about 2-3 times less tolerant to solvents compared to MnP. Methanol and 2-methoxyethanol were extremely inhibitory to LiP. The subtoxic concentrations of acetone were sufficient to remarkably increase the solubility of the model apolar pollutant, anthracene. Each 10% increment in the solvent concentration could be correlated to a 6.4-fold increase in the soluble anthracene concentration. When the peroxidases were incubated in vitro with anthracene as a substrate, acetone was essential for the elimination of anthracene and concomitant formation of anthraquinone. We observed for the first time a H2O2-enhanced oxidation of a polycyclic aromatic hydrocarbon, anthracene, by MnP when enough acetone was used (40% v/v). At acetone concentrations ranging from 5-60%, MnP was also able to oxidize anthracene in the absence of exogenous H2O2 additions but to a lesser extent. The optimum acetone concentration for the LiP-mediated oxidation of anthracene was 10% v/v. The in vitro oxidation rates with semipurified LiP (65 nmol VA min-1 ml-1) and MnP (33 nmol DMP min-1 ml-1) at the optimal solvent concentration was 4.3 and 4.0 mg anthracene l-1 d-1, respectively. The rate of anthracene conversion by LiP was similar to the anthracene dissolution rate in 10% acetone. In contrast, the conversion by MnP was estimated to be several orders of magnitude slower than the dissolution kinetics in 40% acetone.

AB - Lignin peroxidase (LiP) and manganese-dependent peroxidase (MnP) titers in extracellular fluids of five-day-old Bjerkandera sp. strain BOS55 cultures and semipurified preparations from Phanerochaete chrysosporium were evaluated for their tolerance to miscible solvents. MnP assayed with 2,6-dimethoxyphenol (DMP) was found to retain 20% of its normal activity in the presence of 45-58% v/v acetone, 31-34% v/v ethanol, and 29% 2-propanol. LiP assayed with veratryl alcohol (VA) was about 2-3 times less tolerant to solvents compared to MnP. Methanol and 2-methoxyethanol were extremely inhibitory to LiP. The subtoxic concentrations of acetone were sufficient to remarkably increase the solubility of the model apolar pollutant, anthracene. Each 10% increment in the solvent concentration could be correlated to a 6.4-fold increase in the soluble anthracene concentration. When the peroxidases were incubated in vitro with anthracene as a substrate, acetone was essential for the elimination of anthracene and concomitant formation of anthraquinone. We observed for the first time a H2O2-enhanced oxidation of a polycyclic aromatic hydrocarbon, anthracene, by MnP when enough acetone was used (40% v/v). At acetone concentrations ranging from 5-60%, MnP was also able to oxidize anthracene in the absence of exogenous H2O2 additions but to a lesser extent. The optimum acetone concentration for the LiP-mediated oxidation of anthracene was 10% v/v. The in vitro oxidation rates with semipurified LiP (65 nmol VA min-1 ml-1) and MnP (33 nmol DMP min-1 ml-1) at the optimal solvent concentration was 4.3 and 4.0 mg anthracene l-1 d-1, respectively. The rate of anthracene conversion by LiP was similar to the anthracene dissolution rate in 10% acetone. In contrast, the conversion by MnP was estimated to be several orders of magnitude slower than the dissolution kinetics in 40% acetone.

KW - Cosolvent

KW - Dissolution rate

KW - Lignin peroxidase

KW - Manganese-dependent peroxidase

KW - Miscible solvent

KW - Polycyclic aromatic hydrocarbon

KW - Solubility

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