The use of an infectious hypodermal and hematopoietic necrosis virus gene probe serodiagnostic field kit for the screening of candidate specific pathogen-free Penaeus vannamei broodstock

William H. Carr, James N. Sweeney, Linda Nunan, Donald V Lightner, Heidi H. Hirsch, John J. Reddington

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

An experimental non-lethal serodiagnostic kit was evaluated for screening candidate specific pathogen-free (SPF) Penaeus vannamei broodstock under typical field conditions. In this trial, a total of 26 adult P. vannamei was screened for the presence of infectious hypodermal and hematopoietic necrosis virus (IHHNV) using an IHHNV dot blot field kit. The non-radioactive IHHNV in situ hybridization method was used as a standard for comparison. The results showed a significant relationship between the outcome of the dot blot kit and the outcome of the in situ method. In particular, the test results using the experimental kit were consistent with the in situ hybridization method. Based on these findings, the experimental serodiagnostic kit may provide a more rapid, cost-effective diagnostic method than traditional techniques for IHHNV detection.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalAquaculture
Volume147
Issue number1-2
DOIs
StatePublished - Nov 20 1996

Fingerprint

Infectious hematopoietic necrosis virus
breeding stock
Litopenaeus vannamei
virus
pathogen
probe
screening
gene
pathogens
in situ hybridization
genes
methodology
diagnostic techniques
necrosis
method
cost
in situ
testing

Keywords

  • IHHNV
  • Penaeus vannamei
  • Serodiagnostic kit
  • SPF

ASJC Scopus subject areas

  • Aquatic Science

Cite this

The use of an infectious hypodermal and hematopoietic necrosis virus gene probe serodiagnostic field kit for the screening of candidate specific pathogen-free Penaeus vannamei broodstock. / Carr, William H.; Sweeney, James N.; Nunan, Linda; Lightner, Donald V; Hirsch, Heidi H.; Reddington, John J.

In: Aquaculture, Vol. 147, No. 1-2, 20.11.1996, p. 1-8.

Research output: Contribution to journalArticle

@article{3e0299a0386b411d9dfe952d39364130,
title = "The use of an infectious hypodermal and hematopoietic necrosis virus gene probe serodiagnostic field kit for the screening of candidate specific pathogen-free Penaeus vannamei broodstock",
abstract = "An experimental non-lethal serodiagnostic kit was evaluated for screening candidate specific pathogen-free (SPF) Penaeus vannamei broodstock under typical field conditions. In this trial, a total of 26 adult P. vannamei was screened for the presence of infectious hypodermal and hematopoietic necrosis virus (IHHNV) using an IHHNV dot blot field kit. The non-radioactive IHHNV in situ hybridization method was used as a standard for comparison. The results showed a significant relationship between the outcome of the dot blot kit and the outcome of the in situ method. In particular, the test results using the experimental kit were consistent with the in situ hybridization method. Based on these findings, the experimental serodiagnostic kit may provide a more rapid, cost-effective diagnostic method than traditional techniques for IHHNV detection.",
keywords = "IHHNV, Penaeus vannamei, Serodiagnostic kit, SPF",
author = "Carr, {William H.} and Sweeney, {James N.} and Linda Nunan and Lightner, {Donald V} and Hirsch, {Heidi H.} and Reddington, {John J.}",
year = "1996",
month = "11",
day = "20",
doi = "10.1016/S0044-8486(96)01291-4",
language = "English (US)",
volume = "147",
pages = "1--8",
journal = "Aquaculture",
issn = "0044-8486",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - The use of an infectious hypodermal and hematopoietic necrosis virus gene probe serodiagnostic field kit for the screening of candidate specific pathogen-free Penaeus vannamei broodstock

AU - Carr, William H.

AU - Sweeney, James N.

AU - Nunan, Linda

AU - Lightner, Donald V

AU - Hirsch, Heidi H.

AU - Reddington, John J.

PY - 1996/11/20

Y1 - 1996/11/20

N2 - An experimental non-lethal serodiagnostic kit was evaluated for screening candidate specific pathogen-free (SPF) Penaeus vannamei broodstock under typical field conditions. In this trial, a total of 26 adult P. vannamei was screened for the presence of infectious hypodermal and hematopoietic necrosis virus (IHHNV) using an IHHNV dot blot field kit. The non-radioactive IHHNV in situ hybridization method was used as a standard for comparison. The results showed a significant relationship between the outcome of the dot blot kit and the outcome of the in situ method. In particular, the test results using the experimental kit were consistent with the in situ hybridization method. Based on these findings, the experimental serodiagnostic kit may provide a more rapid, cost-effective diagnostic method than traditional techniques for IHHNV detection.

AB - An experimental non-lethal serodiagnostic kit was evaluated for screening candidate specific pathogen-free (SPF) Penaeus vannamei broodstock under typical field conditions. In this trial, a total of 26 adult P. vannamei was screened for the presence of infectious hypodermal and hematopoietic necrosis virus (IHHNV) using an IHHNV dot blot field kit. The non-radioactive IHHNV in situ hybridization method was used as a standard for comparison. The results showed a significant relationship between the outcome of the dot blot kit and the outcome of the in situ method. In particular, the test results using the experimental kit were consistent with the in situ hybridization method. Based on these findings, the experimental serodiagnostic kit may provide a more rapid, cost-effective diagnostic method than traditional techniques for IHHNV detection.

KW - IHHNV

KW - Penaeus vannamei

KW - Serodiagnostic kit

KW - SPF

UR - http://www.scopus.com/inward/record.url?scp=0030595747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030595747&partnerID=8YFLogxK

U2 - 10.1016/S0044-8486(96)01291-4

DO - 10.1016/S0044-8486(96)01291-4

M3 - Article

VL - 147

SP - 1

EP - 8

JO - Aquaculture

JF - Aquaculture

SN - 0044-8486

IS - 1-2

ER -