The use of cultured drosophila cells for studying the microtubule cytoskeleton

Jonathan Nye, Daniel W. Buster, Gregory C. Rogers

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Cultured Drosophila cell lines have been developed into a powerful tool for studying a wide variety of cellular processes. Their ability to be easily and cheaply cultured as well as their susceptibility to protein knockdown via double-stranded RNA-mediated interference (RNAi) has made them the model system of choice for many researchers in the fields of cell biology and functional genomics. Here we describe basic techniques for gene knockdown, transgene expression, preparation for fluorescence microscopy, and centrosome enrichment using cultured Drosophila cells with an emphasis on studying the microtubule cytoskeleton.

Original languageEnglish (US)
Pages (from-to)81-101
Number of pages21
JournalMethods in Molecular Biology
Volume1136
DOIs
StatePublished - Jan 1 2014

Keywords

  • Cell culture
  • Centrosomes
  • Drosophila
  • Kc cells
  • RNAi
  • S2 cells
  • Schneider cells
  • dsRNA-mediated interference

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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