The use of transformed T cell lines for clonal expansion of human B cells from peripheral blood, spleen, and tumor-infiltrating lymphocytes

J. A M Barbuto, E. L. Verastegui, Evan M Hersh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human transformed T cell lines were able to induce polyclonal B cell activation and immunoglobulin (Ig) secretion from peripheral blood mononuclear cells, spleen cells and tumor-infiltrating lymphocytes (TIL). Cells from one of the lines tested, MOT, did not require any exogenous stimuli to induce maximal responses and under similar conditions induced higher levels of response than peripheral blood T cells or other T cell lines. MOT-induced B cell activation and Ig secretion required cell contact and factors present in the MOT culture supernatant. MOT cells induced B cell responses from TIL in the three tumors tested (melanoma, ovarian and colon cancer) and HIV-specific immunoglobulin secretion by spleen cells from an HIV+ patient.

Original languageEnglish (US)
Pages (from-to)115-125
Number of pages11
JournalHybridoma
Volume12
Issue number1
StatePublished - 1993

Fingerprint

Tumor-Infiltrating Lymphocytes
Transformed Cell Line
B-Lymphocytes
Spleen
T-Lymphocytes
Immunoglobulins
Blood Cells
HIV
Cell Line
Ovarian Neoplasms
Colonic Neoplasms
Melanoma
Neoplasms

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

The use of transformed T cell lines for clonal expansion of human B cells from peripheral blood, spleen, and tumor-infiltrating lymphocytes. / Barbuto, J. A M; Verastegui, E. L.; Hersh, Evan M.

In: Hybridoma, Vol. 12, No. 1, 1993, p. 115-125.

Research output: Contribution to journalArticle

@article{11938c95f7d549338d9a811b7005ccd9,
title = "The use of transformed T cell lines for clonal expansion of human B cells from peripheral blood, spleen, and tumor-infiltrating lymphocytes",
abstract = "Human transformed T cell lines were able to induce polyclonal B cell activation and immunoglobulin (Ig) secretion from peripheral blood mononuclear cells, spleen cells and tumor-infiltrating lymphocytes (TIL). Cells from one of the lines tested, MOT, did not require any exogenous stimuli to induce maximal responses and under similar conditions induced higher levels of response than peripheral blood T cells or other T cell lines. MOT-induced B cell activation and Ig secretion required cell contact and factors present in the MOT culture supernatant. MOT cells induced B cell responses from TIL in the three tumors tested (melanoma, ovarian and colon cancer) and HIV-specific immunoglobulin secretion by spleen cells from an HIV+ patient.",
author = "Barbuto, {J. A M} and Verastegui, {E. L.} and Hersh, {Evan M}",
year = "1993",
language = "English (US)",
volume = "12",
pages = "115--125",
journal = "Monoclonal Antibodies in Immunodiagnosis and Immunotherapy",
issn = "2167-9436",
publisher = "Mary Ann Liebert Inc.",
number = "1",

}

TY - JOUR

T1 - The use of transformed T cell lines for clonal expansion of human B cells from peripheral blood, spleen, and tumor-infiltrating lymphocytes

AU - Barbuto, J. A M

AU - Verastegui, E. L.

AU - Hersh, Evan M

PY - 1993

Y1 - 1993

N2 - Human transformed T cell lines were able to induce polyclonal B cell activation and immunoglobulin (Ig) secretion from peripheral blood mononuclear cells, spleen cells and tumor-infiltrating lymphocytes (TIL). Cells from one of the lines tested, MOT, did not require any exogenous stimuli to induce maximal responses and under similar conditions induced higher levels of response than peripheral blood T cells or other T cell lines. MOT-induced B cell activation and Ig secretion required cell contact and factors present in the MOT culture supernatant. MOT cells induced B cell responses from TIL in the three tumors tested (melanoma, ovarian and colon cancer) and HIV-specific immunoglobulin secretion by spleen cells from an HIV+ patient.

AB - Human transformed T cell lines were able to induce polyclonal B cell activation and immunoglobulin (Ig) secretion from peripheral blood mononuclear cells, spleen cells and tumor-infiltrating lymphocytes (TIL). Cells from one of the lines tested, MOT, did not require any exogenous stimuli to induce maximal responses and under similar conditions induced higher levels of response than peripheral blood T cells or other T cell lines. MOT-induced B cell activation and Ig secretion required cell contact and factors present in the MOT culture supernatant. MOT cells induced B cell responses from TIL in the three tumors tested (melanoma, ovarian and colon cancer) and HIV-specific immunoglobulin secretion by spleen cells from an HIV+ patient.

UR - http://www.scopus.com/inward/record.url?scp=0027292044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027292044&partnerID=8YFLogxK

M3 - Article

C2 - 8454298

AN - SCOPUS:0027292044

VL - 12

SP - 115

EP - 125

JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

SN - 2167-9436

IS - 1

ER -