Theory and simulation of isoelectric focusing

T. L. Sounart, P. A. Safier, James C Baygents

Research output: Book/ReportBook

6 Citations (Scopus)

Abstract

IEF is an electrophoretic process that hinges on the mobility versus pH behavior of ampholytes. The basic theory of IEF tells us that separands focus about their pI in a Gaussian distribution. Peak height and variance depend on the competition between diffusion away from the pI and electromigration towards it. Comprehensive simulations of IEF involve the numerical solution to conservation laws for all the relevant amphoteric and ionogenic species in the separations milieu. The conservation relations are non-linearly coupled to the driving electric field and through the complicated mass-action relations. With the increased speed of microprocessors, one-dimensional simulations of the type shown here are now readily managed. The simulations offer the possibility of gaining insight into the detailed dynamics of the unfolding separations process. To see a more comprehensive exposition of IEF simulation results, particularly with regard to pH gradient development, the reader should refer the monograph by Mosher et al.

Original languageEnglish (US)
PublisherUnknown Publisher
Number of pages28
Volume7
EditionC
DOIs
StatePublished - 2005

Publication series

NameSeparation Science and Technology
No.C
Volume7
ISSN (Print)18771718

Fingerprint

Conservation
Electromigration
Gaussian distribution
Hinges
Microprocessor chips
Buffers
Electric fields
Isoelectric Focusing

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Sounart, T. L., Safier, P. A., & Baygents, J. C. (2005). Theory and simulation of isoelectric focusing. (C ed.) (Separation Science and Technology; Vol. 7, No. C). Unknown Publisher. https://doi.org/10.1016/S0149-6395(05)80006-6

Theory and simulation of isoelectric focusing. / Sounart, T. L.; Safier, P. A.; Baygents, James C.

C ed. Unknown Publisher, 2005. 28 p. (Separation Science and Technology; Vol. 7, No. C).

Research output: Book/ReportBook

Sounart, TL, Safier, PA & Baygents, JC 2005, Theory and simulation of isoelectric focusing. Separation Science and Technology, no. C, vol. 7, vol. 7, C edn, Unknown Publisher. https://doi.org/10.1016/S0149-6395(05)80006-6
Sounart TL, Safier PA, Baygents JC. Theory and simulation of isoelectric focusing. C ed. Unknown Publisher, 2005. 28 p. (Separation Science and Technology; C). https://doi.org/10.1016/S0149-6395(05)80006-6
Sounart, T. L. ; Safier, P. A. ; Baygents, James C. / Theory and simulation of isoelectric focusing. C ed. Unknown Publisher, 2005. 28 p. (Separation Science and Technology; C).
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