Thermal enhancement of X-ray-induced DNA crosslinking

G. T. Bowden, M. Kasunic, Anne E Cress

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ionizing radiation appears to crosslink nuclear DNA with chromosomal proteins. Important cellular processes such as transcription and DNA replication are likely to be compromised as a result of the DNA crosslinking. Heat treatment (43°C) of mouse leukemia cells (L1210) before X irradiation (50 Gy) was found to cause a doubling of the radiation-induced DNA crosslinking as measured by alkaline elution technique. By using proteinase K, a very active protease, to eliminate DNA-protein crosslinking in the alkaline elution assay, it was shown that the thermally enhanced DNA crosslinking was attributed to an increase in DNA-protein crosslinking. However, utilizing a protein radiolabel technique under conditions of increased DNA-protein crosslinking, the amount of protein left on the filter in the elution assay was not increased. These data suggest that qualitative rather than large quantitative differences in the crosslinked chromosomal proteins exist between irradiated cells and cells treated with heat prior to irradiation.

Original languageEnglish (US)
Pages (from-to)203-208
Number of pages6
JournalRadiation Research
Volume89
Issue number1
StatePublished - 1982

Fingerprint

crosslinking
X-radiation
deoxyribonucleic acid
Hot Temperature
X-Rays
heat
proteins
augmentation
DNA
elution
Proteins
x rays
irradiation
Leukemia L1210
Endopeptidase K
protease
assays
DNA replication
cells
leukemias

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Thermal enhancement of X-ray-induced DNA crosslinking. / Bowden, G. T.; Kasunic, M.; Cress, Anne E.

In: Radiation Research, Vol. 89, No. 1, 1982, p. 203-208.

Research output: Contribution to journalArticle

Bowden, GT, Kasunic, M & Cress, AE 1982, 'Thermal enhancement of X-ray-induced DNA crosslinking', Radiation Research, vol. 89, no. 1, pp. 203-208.
Bowden, G. T. ; Kasunic, M. ; Cress, Anne E. / Thermal enhancement of X-ray-induced DNA crosslinking. In: Radiation Research. 1982 ; Vol. 89, No. 1. pp. 203-208.
@article{1c9d6f7481044626ad1c9152cb16158e,
title = "Thermal enhancement of X-ray-induced DNA crosslinking",
abstract = "Ionizing radiation appears to crosslink nuclear DNA with chromosomal proteins. Important cellular processes such as transcription and DNA replication are likely to be compromised as a result of the DNA crosslinking. Heat treatment (43°C) of mouse leukemia cells (L1210) before X irradiation (50 Gy) was found to cause a doubling of the radiation-induced DNA crosslinking as measured by alkaline elution technique. By using proteinase K, a very active protease, to eliminate DNA-protein crosslinking in the alkaline elution assay, it was shown that the thermally enhanced DNA crosslinking was attributed to an increase in DNA-protein crosslinking. However, utilizing a protein radiolabel technique under conditions of increased DNA-protein crosslinking, the amount of protein left on the filter in the elution assay was not increased. These data suggest that qualitative rather than large quantitative differences in the crosslinked chromosomal proteins exist between irradiated cells and cells treated with heat prior to irradiation.",
author = "Bowden, {G. T.} and M. Kasunic and Cress, {Anne E}",
year = "1982",
language = "English (US)",
volume = "89",
pages = "203--208",
journal = "Radiation Research",
issn = "0033-7587",
publisher = "Radiation Research Society",
number = "1",

}

TY - JOUR

T1 - Thermal enhancement of X-ray-induced DNA crosslinking

AU - Bowden, G. T.

AU - Kasunic, M.

AU - Cress, Anne E

PY - 1982

Y1 - 1982

N2 - Ionizing radiation appears to crosslink nuclear DNA with chromosomal proteins. Important cellular processes such as transcription and DNA replication are likely to be compromised as a result of the DNA crosslinking. Heat treatment (43°C) of mouse leukemia cells (L1210) before X irradiation (50 Gy) was found to cause a doubling of the radiation-induced DNA crosslinking as measured by alkaline elution technique. By using proteinase K, a very active protease, to eliminate DNA-protein crosslinking in the alkaline elution assay, it was shown that the thermally enhanced DNA crosslinking was attributed to an increase in DNA-protein crosslinking. However, utilizing a protein radiolabel technique under conditions of increased DNA-protein crosslinking, the amount of protein left on the filter in the elution assay was not increased. These data suggest that qualitative rather than large quantitative differences in the crosslinked chromosomal proteins exist between irradiated cells and cells treated with heat prior to irradiation.

AB - Ionizing radiation appears to crosslink nuclear DNA with chromosomal proteins. Important cellular processes such as transcription and DNA replication are likely to be compromised as a result of the DNA crosslinking. Heat treatment (43°C) of mouse leukemia cells (L1210) before X irradiation (50 Gy) was found to cause a doubling of the radiation-induced DNA crosslinking as measured by alkaline elution technique. By using proteinase K, a very active protease, to eliminate DNA-protein crosslinking in the alkaline elution assay, it was shown that the thermally enhanced DNA crosslinking was attributed to an increase in DNA-protein crosslinking. However, utilizing a protein radiolabel technique under conditions of increased DNA-protein crosslinking, the amount of protein left on the filter in the elution assay was not increased. These data suggest that qualitative rather than large quantitative differences in the crosslinked chromosomal proteins exist between irradiated cells and cells treated with heat prior to irradiation.

UR - http://www.scopus.com/inward/record.url?scp=0020084298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020084298&partnerID=8YFLogxK

M3 - Article

C2 - 7038740

AN - SCOPUS:0020084298

VL - 89

SP - 203

EP - 208

JO - Radiation Research

JF - Radiation Research

SN - 0033-7587

IS - 1

ER -