Thermal enhancement of X-ray-induced DNA crosslinking

G. T. Bowden, M. Kasunic, A. E. Cress

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Ionizing radiation appears to crosslink nuclear DNA with chromosomal proteins. Important cellular processes such as transcription and DNA replication are likely to be compromised as a result of the DNA crosslinking. Heat treatment (43°C) of mouse leukemia cells (L1210) before X irradiation (50 Gy) was found to cause a doubling of the radiation-induced DNA crosslinking as measured by alkaline elution technique. By using proteinase K, a very active protease, to eliminate DNA-protein crosslinking in the alkaline elution assay, it was shown that the thermally enhanced DNA crosslinking was attributed to an increase in DNA-protein crosslinking. However, utilizing a protein radiolabel technique under conditions of increased DNA-protein crosslinking, the amount of protein left on the filter in the elution assay was not increased. These data suggest that qualitative rather than large quantitative differences in the crosslinked chromosomal proteins exist between irradiated cells and cells treated with heat prior to irradiation.

Original languageEnglish (US)
Pages (from-to)203-208
Number of pages6
JournalRadiation Research
Volume89
Issue number1
DOIs
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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