Thioredoxin peroxidase-1 (peroxiredoxin-1) is increased in thioredoxin-1 transfected cells and results in enhanced protection against apoptosis caused by hydrogen peroxide but not by other agents including dexamethasone, etoposide, and doxorubicin

M. I. Berggren, B. Husbeck, B. Samulitis, Amanda F Baker, A. Gallegos, G. Powis

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Abstract

Thioredoxin-1 (Trx-1) is a small redox oncoprotein whose expression is increased in a number of human primary cancers where it is associated with aggressive tumor growth, inhibition of apoptosis and decreased patient survival. We report that Trx-1-transfected MCF-7 human breast cancer cells have increased expression of thioredoxin peroxidase-1 (TrxP-1) a peroxiredoxin family member that scavenges H2O2 using Trx-1 as a source of reducing equivalents. Our work shows that TrxP-1 is more effective than selenium-dependent glutathione peroxidase in protecting cells against H2O2 damage. Transfection of mouse WEHI7.2 lymphoma cells with human TrxP-1 or TrxP-2, but not TrxP-4, protects the cells against H2O2 induced apoptosis but does not protect against apoptosis induced by dexamethasone, etoposide, or doxorubicin. The results show that an increase in TrxP-1 expression contributes to the protection against H2O2 induced apoptosis caused by Trx-1, but does not protect against apoptosis induced by other agents.

Original languageEnglish (US)
Pages (from-to)103-109
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume392
Issue number1
DOIs
Publication statusPublished - Aug 1 2001

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Keywords

  • Apoptosis
  • Hydrogen peroxide
  • Selenium
  • Thioredoxin peroxidase
  • Thioredoxin-1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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