Thoracic aorta occlusion-reperfusion decreases hemostasis as assessed by thromboelastography in rabbits

Vance G Nielsen, Brian T. Geary

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Perioperative hemorrhage and thrombosis are serious complications associated with major vascular surgery. We hypothesized that thoracic aortic occlusion-reperfusion in rabbits would adversely affect hemostasis as assessed by thromboelastographic variables (reaction time, α angle and G [a measure of clot strength]). Isoflurane-anesthetized rabbits underwent either sham operation (n = 10) or 30 min of aortic occlusion followed by 90 min of reperfusion (n = 10). Blood samples (350/μL) were exposed to 10 μL of either 0.9% NaCl or cytochalasin D (a platelet inhibitor, 10 μM final concentration) and analyzed for 1 h by using thromboelastography after 30 min of postpreparation equilibration and at 30 and 90 min of reperfusion. Aortic occlusion-reperfusion resulted in a significant (P < 0.05) increase in reaction time, decrease in α angle, and decrease in G at 30 and 90 min of reperfusion compared with the sham-operated group. The decrease in hemostatic function after aortic occlusion-reperfusion was observed to the same degree in samples with or without platelet inhibition. There were no significant differences in platelet concentration between the sham-operated and aortic occlusion-reperfusion groups. Aortic occlusion-reperfusion decreased hemostatic function in rabbits primarily by decreasing the coagulation factor-dependent, platelet-independent contribution to clotting.

Original languageEnglish (US)
Pages (from-to)517-521
Number of pages5
JournalAnesthesia and Analgesia
Volume91
Issue number3
StatePublished - 2000
Externally publishedYes

Fingerprint

Thrombelastography
Hemostasis
Thoracic Aorta
Reperfusion
Rabbits
Blood Platelets
Hemostatics
Cytochalasin D
Blood Coagulation Factors
Isoflurane
Platelet Aggregation Inhibitors
Blood Vessels
Thrombosis
Thorax
Hemorrhage

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Thoracic aorta occlusion-reperfusion decreases hemostasis as assessed by thromboelastography in rabbits. / Nielsen, Vance G; Geary, Brian T.

In: Anesthesia and Analgesia, Vol. 91, No. 3, 2000, p. 517-521.

Research output: Contribution to journalArticle

@article{fcc0841fe00441768c30feb33cad5265,
title = "Thoracic aorta occlusion-reperfusion decreases hemostasis as assessed by thromboelastography in rabbits",
abstract = "Perioperative hemorrhage and thrombosis are serious complications associated with major vascular surgery. We hypothesized that thoracic aortic occlusion-reperfusion in rabbits would adversely affect hemostasis as assessed by thromboelastographic variables (reaction time, α angle and G [a measure of clot strength]). Isoflurane-anesthetized rabbits underwent either sham operation (n = 10) or 30 min of aortic occlusion followed by 90 min of reperfusion (n = 10). Blood samples (350/μL) were exposed to 10 μL of either 0.9{\%} NaCl or cytochalasin D (a platelet inhibitor, 10 μM final concentration) and analyzed for 1 h by using thromboelastography after 30 min of postpreparation equilibration and at 30 and 90 min of reperfusion. Aortic occlusion-reperfusion resulted in a significant (P < 0.05) increase in reaction time, decrease in α angle, and decrease in G at 30 and 90 min of reperfusion compared with the sham-operated group. The decrease in hemostatic function after aortic occlusion-reperfusion was observed to the same degree in samples with or without platelet inhibition. There were no significant differences in platelet concentration between the sham-operated and aortic occlusion-reperfusion groups. Aortic occlusion-reperfusion decreased hemostatic function in rabbits primarily by decreasing the coagulation factor-dependent, platelet-independent contribution to clotting.",
author = "Nielsen, {Vance G} and Geary, {Brian T.}",
year = "2000",
language = "English (US)",
volume = "91",
pages = "517--521",
journal = "Anesthesia and Analgesia",
issn = "0003-2999",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Thoracic aorta occlusion-reperfusion decreases hemostasis as assessed by thromboelastography in rabbits

AU - Nielsen, Vance G

AU - Geary, Brian T.

PY - 2000

Y1 - 2000

N2 - Perioperative hemorrhage and thrombosis are serious complications associated with major vascular surgery. We hypothesized that thoracic aortic occlusion-reperfusion in rabbits would adversely affect hemostasis as assessed by thromboelastographic variables (reaction time, α angle and G [a measure of clot strength]). Isoflurane-anesthetized rabbits underwent either sham operation (n = 10) or 30 min of aortic occlusion followed by 90 min of reperfusion (n = 10). Blood samples (350/μL) were exposed to 10 μL of either 0.9% NaCl or cytochalasin D (a platelet inhibitor, 10 μM final concentration) and analyzed for 1 h by using thromboelastography after 30 min of postpreparation equilibration and at 30 and 90 min of reperfusion. Aortic occlusion-reperfusion resulted in a significant (P < 0.05) increase in reaction time, decrease in α angle, and decrease in G at 30 and 90 min of reperfusion compared with the sham-operated group. The decrease in hemostatic function after aortic occlusion-reperfusion was observed to the same degree in samples with or without platelet inhibition. There were no significant differences in platelet concentration between the sham-operated and aortic occlusion-reperfusion groups. Aortic occlusion-reperfusion decreased hemostatic function in rabbits primarily by decreasing the coagulation factor-dependent, platelet-independent contribution to clotting.

AB - Perioperative hemorrhage and thrombosis are serious complications associated with major vascular surgery. We hypothesized that thoracic aortic occlusion-reperfusion in rabbits would adversely affect hemostasis as assessed by thromboelastographic variables (reaction time, α angle and G [a measure of clot strength]). Isoflurane-anesthetized rabbits underwent either sham operation (n = 10) or 30 min of aortic occlusion followed by 90 min of reperfusion (n = 10). Blood samples (350/μL) were exposed to 10 μL of either 0.9% NaCl or cytochalasin D (a platelet inhibitor, 10 μM final concentration) and analyzed for 1 h by using thromboelastography after 30 min of postpreparation equilibration and at 30 and 90 min of reperfusion. Aortic occlusion-reperfusion resulted in a significant (P < 0.05) increase in reaction time, decrease in α angle, and decrease in G at 30 and 90 min of reperfusion compared with the sham-operated group. The decrease in hemostatic function after aortic occlusion-reperfusion was observed to the same degree in samples with or without platelet inhibition. There were no significant differences in platelet concentration between the sham-operated and aortic occlusion-reperfusion groups. Aortic occlusion-reperfusion decreased hemostatic function in rabbits primarily by decreasing the coagulation factor-dependent, platelet-independent contribution to clotting.

UR - http://www.scopus.com/inward/record.url?scp=0033855715&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033855715&partnerID=8YFLogxK

M3 - Article

C2 - 10960368

AN - SCOPUS:0033855715

VL - 91

SP - 517

EP - 521

JO - Anesthesia and Analgesia

JF - Anesthesia and Analgesia

SN - 0003-2999

IS - 3

ER -