Three cadherin alleles associated with resistance to Bacillus thuringiensis in pink bollworm

Shai Morin, Robert W. Biggs, Mark S. Sisterson, Laura Shriver, Christa Ellers-Kirk, Dawn Higginson, Daniel Holley, Linda J. Gahan, David G. Heckel, Yves Carrière, Timothy J. Dennehy, Judith K. Brown, Bruce E. Tabashnik

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332 Scopus citations

Abstract

Evolution of resistance by pests is the main threat to long-term insect control by transgenic crops that produce Bacillus thuringiensis (Bt) toxins. Because inheritance of resistance to the Bt toxins in transgenic crops is typically recessive, DNA-based screening for resistance alleles in heterozygotes is potentially much more efficient than detection of resistant homozygotes with bioassays. Such screening, however, requires knowledge of the resistance alleles in field populations of pests that are associated with survival on Bt crops. Here we report that field populations of pink bollworm (Pectinophora gossypiella), a major cotton pest, harbored three mutant alleles of a cadherin-encoding gene linked with resistance to Bt toxin Cry1Ac and survival on transgenic Bt cotton. Each of the three resistance alleles has a deletion expected to eliminate at least eight amino acids upstream of the putative toxin-binding region of the cadherin protein. Larvae with two resistance alleles in any combination were resistant, whereas those with one or none were susceptible to Cry1Ac. Together with previous evidence, the results reported here identify the cadherin gene as a leading target for DNA-based screening of resistance to Bt crops in lepidopteran pests.

Original languageEnglish (US)
Pages (from-to)5004-5009
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number9
DOIs
StatePublished - Apr 29 2003

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    Morin, S., Biggs, R. W., Sisterson, M. S., Shriver, L., Ellers-Kirk, C., Higginson, D., Holley, D., Gahan, L. J., Heckel, D. G., Carrière, Y., Dennehy, T. J., Brown, J. K., & Tabashnik, B. E. (2003). Three cadherin alleles associated with resistance to Bacillus thuringiensis in pink bollworm. Proceedings of the National Academy of Sciences of the United States of America, 100(9), 5004-5009. https://doi.org/10.1073/pnas.0831036100