Three-dimensional architecture of collecting ducts, loops of Henle, and blood vessels in the renal papilla

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Three-dimensional architecture of vasculature and nephrons in rat renal papilla was assessed by digital reconstruction. Descending vasa recta (DVR), ascending vasa recta (AVR), descending thin limbs (DTLs), ascending thin limbs (ATLs), and collecting ducts (CDs) were identified with antibodies against segment-specific proteins. DTLs are distributed nonuniformly in transverse sections of papilla, but lateral compartmentation between DTLs and CD clusters that occurs in outer IM makes no contribution to concentrating mechanism in papilla. ATLs are distributed nearly uniformly throughout IM. Vasa recta within ∼2 mm of the papilla tip are primarily fenestrated vessels; therefore, AVR and DVR can only be determined by blood flow direction. CDs within ∼500 μm of the papilla tip have nearly 100% greater circumference than CDs within first 1-2 mm below the IM base. Return of water to general circulation from deep papillary CDs appears to be facilitated by a 150% increase in the number of AVR closely abutting these CDs. Consequently, average fractional CD surface area abutting AVR is 0.61, about the same as that (0.54) for smaller CDs that lie near the IM base. Interstitial nodal compartments, bounded by CDs, ATLs, and AVR, surround CDs along the axis of the IM. Fewer ATLs exist in the final 1 mm, as there are fewer loops and the number of these nodal arrangements is therefore reduced. However, tips of many of those loops reaching this area have bends with 50-100% greater transverse lengths than bends of loops near the IM base. This may be significant for solute movement out of loop bends.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume293
Issue number3
DOIs
StatePublished - Sep 2007

Fingerprint

Loop of Henle
Rectum
Blood Vessels
Extremities
Kidney
Nephrons
Water
Antibodies

Keywords

  • αB crystallin
  • Aquaporin
  • ClC-K
  • Concentrating mechanism
  • Countercurrent multiplier
  • Three-dimensional reconstruction

ASJC Scopus subject areas

  • Physiology

Cite this

@article{cb0f9934c001406c8bb45f1b234f7203,
title = "Three-dimensional architecture of collecting ducts, loops of Henle, and blood vessels in the renal papilla",
abstract = "Three-dimensional architecture of vasculature and nephrons in rat renal papilla was assessed by digital reconstruction. Descending vasa recta (DVR), ascending vasa recta (AVR), descending thin limbs (DTLs), ascending thin limbs (ATLs), and collecting ducts (CDs) were identified with antibodies against segment-specific proteins. DTLs are distributed nonuniformly in transverse sections of papilla, but lateral compartmentation between DTLs and CD clusters that occurs in outer IM makes no contribution to concentrating mechanism in papilla. ATLs are distributed nearly uniformly throughout IM. Vasa recta within ∼2 mm of the papilla tip are primarily fenestrated vessels; therefore, AVR and DVR can only be determined by blood flow direction. CDs within ∼500 μm of the papilla tip have nearly 100{\%} greater circumference than CDs within first 1-2 mm below the IM base. Return of water to general circulation from deep papillary CDs appears to be facilitated by a 150{\%} increase in the number of AVR closely abutting these CDs. Consequently, average fractional CD surface area abutting AVR is 0.61, about the same as that (0.54) for smaller CDs that lie near the IM base. Interstitial nodal compartments, bounded by CDs, ATLs, and AVR, surround CDs along the axis of the IM. Fewer ATLs exist in the final 1 mm, as there are fewer loops and the number of these nodal arrangements is therefore reduced. However, tips of many of those loops reaching this area have bends with 50-100{\%} greater transverse lengths than bends of loops near the IM base. This may be significant for solute movement out of loop bends.",
keywords = "αB crystallin, Aquaporin, ClC-K, Concentrating mechanism, Countercurrent multiplier, Three-dimensional reconstruction",
author = "Pannabecker, {Thomas L} and Dantzler, {William H}",
year = "2007",
month = "9",
doi = "10.1152/ajprenal.00231.2007",
language = "English (US)",
volume = "293",
journal = "American Journal of Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "3",

}

TY - JOUR

T1 - Three-dimensional architecture of collecting ducts, loops of Henle, and blood vessels in the renal papilla

AU - Pannabecker, Thomas L

AU - Dantzler, William H

PY - 2007/9

Y1 - 2007/9

N2 - Three-dimensional architecture of vasculature and nephrons in rat renal papilla was assessed by digital reconstruction. Descending vasa recta (DVR), ascending vasa recta (AVR), descending thin limbs (DTLs), ascending thin limbs (ATLs), and collecting ducts (CDs) were identified with antibodies against segment-specific proteins. DTLs are distributed nonuniformly in transverse sections of papilla, but lateral compartmentation between DTLs and CD clusters that occurs in outer IM makes no contribution to concentrating mechanism in papilla. ATLs are distributed nearly uniformly throughout IM. Vasa recta within ∼2 mm of the papilla tip are primarily fenestrated vessels; therefore, AVR and DVR can only be determined by blood flow direction. CDs within ∼500 μm of the papilla tip have nearly 100% greater circumference than CDs within first 1-2 mm below the IM base. Return of water to general circulation from deep papillary CDs appears to be facilitated by a 150% increase in the number of AVR closely abutting these CDs. Consequently, average fractional CD surface area abutting AVR is 0.61, about the same as that (0.54) for smaller CDs that lie near the IM base. Interstitial nodal compartments, bounded by CDs, ATLs, and AVR, surround CDs along the axis of the IM. Fewer ATLs exist in the final 1 mm, as there are fewer loops and the number of these nodal arrangements is therefore reduced. However, tips of many of those loops reaching this area have bends with 50-100% greater transverse lengths than bends of loops near the IM base. This may be significant for solute movement out of loop bends.

AB - Three-dimensional architecture of vasculature and nephrons in rat renal papilla was assessed by digital reconstruction. Descending vasa recta (DVR), ascending vasa recta (AVR), descending thin limbs (DTLs), ascending thin limbs (ATLs), and collecting ducts (CDs) were identified with antibodies against segment-specific proteins. DTLs are distributed nonuniformly in transverse sections of papilla, but lateral compartmentation between DTLs and CD clusters that occurs in outer IM makes no contribution to concentrating mechanism in papilla. ATLs are distributed nearly uniformly throughout IM. Vasa recta within ∼2 mm of the papilla tip are primarily fenestrated vessels; therefore, AVR and DVR can only be determined by blood flow direction. CDs within ∼500 μm of the papilla tip have nearly 100% greater circumference than CDs within first 1-2 mm below the IM base. Return of water to general circulation from deep papillary CDs appears to be facilitated by a 150% increase in the number of AVR closely abutting these CDs. Consequently, average fractional CD surface area abutting AVR is 0.61, about the same as that (0.54) for smaller CDs that lie near the IM base. Interstitial nodal compartments, bounded by CDs, ATLs, and AVR, surround CDs along the axis of the IM. Fewer ATLs exist in the final 1 mm, as there are fewer loops and the number of these nodal arrangements is therefore reduced. However, tips of many of those loops reaching this area have bends with 50-100% greater transverse lengths than bends of loops near the IM base. This may be significant for solute movement out of loop bends.

KW - αB crystallin

KW - Aquaporin

KW - ClC-K

KW - Concentrating mechanism

KW - Countercurrent multiplier

KW - Three-dimensional reconstruction

UR - http://www.scopus.com/inward/record.url?scp=34548627230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548627230&partnerID=8YFLogxK

U2 - 10.1152/ajprenal.00231.2007

DO - 10.1152/ajprenal.00231.2007

M3 - Article

C2 - 17609288

AN - SCOPUS:34548627230

VL - 293

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6143

IS - 3

ER -