Three-dimensional reconstruction of rat dermal blood vessels in vivo

Jennifer K Barton, Joseph A. Izatt, Manish D. Kulkarni, Ashley J. Welch

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We performed imaging and reconstruction of dermal and subdermal blood vessels in a rat skin flap window model. The window model consists of a double thickness of dorsal skin which is sutured to a holding fixture. A 1 cm circle of skin is removed from one thickness, exposing the dermal blood vessels of the opposing side. An optical coherence tomography system operating at 1310 nm was used to image the blood vessels. A series of transverse images of the window model characterized sections of tissue. Off-the-shelf software for desktop and workstation computers was used to preprocess the images, identify and reconstruct blood vessels, and to extract parameters such depth, diameter, and percent volume of blood vessels. Such parameters may be of interest in developing improved treatments for vascular disorders such as port wine stains.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Pages266-275
Number of pages10
Volume2970
DOIs
Publication statusPublished - 1997
Externally publishedYes
EventLasers in Surgery: Advanced Characterization, Therapeutics, and Systems VII - San Jose, CA, United States
Duration: Feb 8 1997Feb 8 1997

Other

OtherLasers in Surgery: Advanced Characterization, Therapeutics, and Systems VII
CountryUnited States
CitySan Jose, CA
Period2/8/972/8/97

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Keywords

  • Image analysis
  • Optical coherence tomography
  • Port wine stains

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Barton, J. K., Izatt, J. A., Kulkarni, M. D., & Welch, A. J. (1997). Three-dimensional reconstruction of rat dermal blood vessels in vivo. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2970, pp. 266-275) https://doi.org/10.1117/12.275053