Thrombelastographic characterization of coagulation/fibrinolysis in horses: Role of carboxyheme and metheme states

Christine S. Olver, Vance G Nielsen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Carboxyheme and metheme states modulate hemostasis in humans and other species. Further, carbon monoxide and/or nitric oxide production increase in inflammatory disorders involving the gastrointestinal tract, with associated hypercoagulability or hypocoagulability. In particular, the horse suffers both thrombotic or coagulopathic complications during acute gastrointestinal disease. This investigation characterized the thrombelastographic response to carboxyheme (via CORM-2) or metheme (via phenylhydroxylamine, PHA) states without/with addition of tissue type plasminogen activator. Citrated plasma was obtained from 14 normal mares and three horses with enteritis. In normal horses, a carboxyheme state did not enhance the velocity of clot growth and minimally enhanced clot strength (9%). In contrast, a metheme state was associated with a decrease in the velocity of clot formation (54%) and clot strength (47%). During fibrinolysis, a carboxyheme state significantly decreased the onset (113%) and velocity (27%) of fibrinolysis; however, in contrast, a metheme state more markedly increased the onset (84%) and velocity (133%) of fibrinolysis. These data support a carbon monoxide-dominant modulation of hemostasis in normal horses. In contrast, an increase in the severity of acute gastrointestinal disease was associated with a likely nitric oxide-mediated, metheme state-induced hypocoagulable/hyperfibrinolytic state. Additional investigation is warranted to determine the role played by carbon monoxide and nitric oxide in equine thrombotic and coagulopathic disease.

Original languageEnglish (US)
Pages (from-to)273-278
Number of pages6
JournalBlood Coagulation and Fibrinolysis
Volume24
Issue number3
DOIs
StatePublished - Apr 2013

Fingerprint

Fibrinolysis
Horses
Carbon Monoxide
Nitric Oxide
Gastrointestinal Diseases
Acute Disease
Hemostasis
Thrombophilia
Enteritis
Tissue Plasminogen Activator
Gastrointestinal Tract
Growth

Keywords

  • carbon monoxide
  • enteritis
  • horses
  • nitric oxide
  • thrombelastography

ASJC Scopus subject areas

  • Hematology

Cite this

Thrombelastographic characterization of coagulation/fibrinolysis in horses : Role of carboxyheme and metheme states. / Olver, Christine S.; Nielsen, Vance G.

In: Blood Coagulation and Fibrinolysis, Vol. 24, No. 3, 04.2013, p. 273-278.

Research output: Contribution to journalArticle

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