Thrombin inhibits active sodium-potassium transport in porcine lens

Mansim C. Okafor, William L. Dean, Nicholas A Delamere

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Purpose. Although thrombin is best known for its role in blood coagulation, it has been reported to change the activity of ion motive ATPases in some tissues. In the present study, experiments were conducted to determine the influence of thrombin on active sodium-potassium transport in porcine lenses. Methods. Ouabain-sensitive potassium (86Rb) uptake by intact porcine lenses was used as an index of Na,K-ATPase-mediated active sodium-potassium transport. Na,K-ATPase activity was measured by determining ouabain-sensitive ATP hydrolysis in isolated membrane material. Results. In the presence of thrombin (1 unit/ml) the rate of ouabain-sensitive potassium (86Rb) uptake was reduced by 40% to 60%, but ouabain-insensitive potassium (86Rb) uptake was unchanged. The inhibitory effect of thrombin on ouabain- sensitive potassium (86Rb) uptake was suppressed in the presence of hirudin (an antagonist for thrombin receptors) but persisted in the presence of amphotericin B (a pseudo ionophore that effectively clamps plasma membrane sodium permeability at a high value). Enzyme measurements showed ouabain- sensitive ATP hydrolysis (Na,K-ATPase activity) was significantly inhibited in membrane material isolated from the capsule-epithelium of lenses, which had been pretreated with thrombin for 30 minutes. However, thrombin failed to exert a direct inhibitory effect on Na,K-ATPase activity when added directly to membrane fragments isolated from the epithelium of control (nonincubated) lenses. Both genistein and herbimycin (tyrosine kinase inhibitors) suppressed the effect of thrombin on the 86Rb uptake response. Results from Western blot studies suggested that tyrosine kinases are activated in the epithelium of lenses exposed to thrombin. Conclusions. The results suggest the inhibitory effect of thrombin on lens active sodium-potassium transport could involve the activation of a receptor-second-messenger mechanism in intact lens cells. The response appears to involve a tyrosine kinase-mediated step. The functional significance of the thrombin-mediated change of lens active sodium-potassium transport is unclear since appreciable amounts of thrombin may only be presented to the lens during instances of blood-aqueous-barrier breakdown. It is possible that lens receptors are functionally activated by other proteases, possibly cathepsins, which may enter aqueous humor from the ciliary body.

Original languageEnglish (US)
Pages (from-to)2033-2038
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume40
Issue number9
StatePublished - Aug 1999
Externally publishedYes

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Thrombin
Lenses
Potassium
Swine
Sodium
Ouabain
Protein-Tyrosine Kinases
Epithelium
Membranes
Hydrolysis
Adenosine Triphosphate
Blood-Aqueous Barrier
Thrombin Receptors
Hirudins
Cathepsins
Ciliary Body
Aqueous Humor
Genistein
Ionophores
Second Messenger Systems

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Thrombin inhibits active sodium-potassium transport in porcine lens. / Okafor, Mansim C.; Dean, William L.; Delamere, Nicholas A.

In: Investigative Ophthalmology and Visual Science, Vol. 40, No. 9, 08.1999, p. 2033-2038.

Research output: Contribution to journalArticle

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title = "Thrombin inhibits active sodium-potassium transport in porcine lens",
abstract = "Purpose. Although thrombin is best known for its role in blood coagulation, it has been reported to change the activity of ion motive ATPases in some tissues. In the present study, experiments were conducted to determine the influence of thrombin on active sodium-potassium transport in porcine lenses. Methods. Ouabain-sensitive potassium (86Rb) uptake by intact porcine lenses was used as an index of Na,K-ATPase-mediated active sodium-potassium transport. Na,K-ATPase activity was measured by determining ouabain-sensitive ATP hydrolysis in isolated membrane material. Results. In the presence of thrombin (1 unit/ml) the rate of ouabain-sensitive potassium (86Rb) uptake was reduced by 40{\%} to 60{\%}, but ouabain-insensitive potassium (86Rb) uptake was unchanged. The inhibitory effect of thrombin on ouabain- sensitive potassium (86Rb) uptake was suppressed in the presence of hirudin (an antagonist for thrombin receptors) but persisted in the presence of amphotericin B (a pseudo ionophore that effectively clamps plasma membrane sodium permeability at a high value). Enzyme measurements showed ouabain- sensitive ATP hydrolysis (Na,K-ATPase activity) was significantly inhibited in membrane material isolated from the capsule-epithelium of lenses, which had been pretreated with thrombin for 30 minutes. However, thrombin failed to exert a direct inhibitory effect on Na,K-ATPase activity when added directly to membrane fragments isolated from the epithelium of control (nonincubated) lenses. Both genistein and herbimycin (tyrosine kinase inhibitors) suppressed the effect of thrombin on the 86Rb uptake response. Results from Western blot studies suggested that tyrosine kinases are activated in the epithelium of lenses exposed to thrombin. Conclusions. The results suggest the inhibitory effect of thrombin on lens active sodium-potassium transport could involve the activation of a receptor-second-messenger mechanism in intact lens cells. The response appears to involve a tyrosine kinase-mediated step. The functional significance of the thrombin-mediated change of lens active sodium-potassium transport is unclear since appreciable amounts of thrombin may only be presented to the lens during instances of blood-aqueous-barrier breakdown. It is possible that lens receptors are functionally activated by other proteases, possibly cathepsins, which may enter aqueous humor from the ciliary body.",
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T1 - Thrombin inhibits active sodium-potassium transport in porcine lens

AU - Okafor, Mansim C.

AU - Dean, William L.

AU - Delamere, Nicholas A

PY - 1999/8

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N2 - Purpose. Although thrombin is best known for its role in blood coagulation, it has been reported to change the activity of ion motive ATPases in some tissues. In the present study, experiments were conducted to determine the influence of thrombin on active sodium-potassium transport in porcine lenses. Methods. Ouabain-sensitive potassium (86Rb) uptake by intact porcine lenses was used as an index of Na,K-ATPase-mediated active sodium-potassium transport. Na,K-ATPase activity was measured by determining ouabain-sensitive ATP hydrolysis in isolated membrane material. Results. In the presence of thrombin (1 unit/ml) the rate of ouabain-sensitive potassium (86Rb) uptake was reduced by 40% to 60%, but ouabain-insensitive potassium (86Rb) uptake was unchanged. The inhibitory effect of thrombin on ouabain- sensitive potassium (86Rb) uptake was suppressed in the presence of hirudin (an antagonist for thrombin receptors) but persisted in the presence of amphotericin B (a pseudo ionophore that effectively clamps plasma membrane sodium permeability at a high value). Enzyme measurements showed ouabain- sensitive ATP hydrolysis (Na,K-ATPase activity) was significantly inhibited in membrane material isolated from the capsule-epithelium of lenses, which had been pretreated with thrombin for 30 minutes. However, thrombin failed to exert a direct inhibitory effect on Na,K-ATPase activity when added directly to membrane fragments isolated from the epithelium of control (nonincubated) lenses. Both genistein and herbimycin (tyrosine kinase inhibitors) suppressed the effect of thrombin on the 86Rb uptake response. Results from Western blot studies suggested that tyrosine kinases are activated in the epithelium of lenses exposed to thrombin. Conclusions. The results suggest the inhibitory effect of thrombin on lens active sodium-potassium transport could involve the activation of a receptor-second-messenger mechanism in intact lens cells. The response appears to involve a tyrosine kinase-mediated step. The functional significance of the thrombin-mediated change of lens active sodium-potassium transport is unclear since appreciable amounts of thrombin may only be presented to the lens during instances of blood-aqueous-barrier breakdown. It is possible that lens receptors are functionally activated by other proteases, possibly cathepsins, which may enter aqueous humor from the ciliary body.

AB - Purpose. Although thrombin is best known for its role in blood coagulation, it has been reported to change the activity of ion motive ATPases in some tissues. In the present study, experiments were conducted to determine the influence of thrombin on active sodium-potassium transport in porcine lenses. Methods. Ouabain-sensitive potassium (86Rb) uptake by intact porcine lenses was used as an index of Na,K-ATPase-mediated active sodium-potassium transport. Na,K-ATPase activity was measured by determining ouabain-sensitive ATP hydrolysis in isolated membrane material. Results. In the presence of thrombin (1 unit/ml) the rate of ouabain-sensitive potassium (86Rb) uptake was reduced by 40% to 60%, but ouabain-insensitive potassium (86Rb) uptake was unchanged. The inhibitory effect of thrombin on ouabain- sensitive potassium (86Rb) uptake was suppressed in the presence of hirudin (an antagonist for thrombin receptors) but persisted in the presence of amphotericin B (a pseudo ionophore that effectively clamps plasma membrane sodium permeability at a high value). Enzyme measurements showed ouabain- sensitive ATP hydrolysis (Na,K-ATPase activity) was significantly inhibited in membrane material isolated from the capsule-epithelium of lenses, which had been pretreated with thrombin for 30 minutes. However, thrombin failed to exert a direct inhibitory effect on Na,K-ATPase activity when added directly to membrane fragments isolated from the epithelium of control (nonincubated) lenses. Both genistein and herbimycin (tyrosine kinase inhibitors) suppressed the effect of thrombin on the 86Rb uptake response. Results from Western blot studies suggested that tyrosine kinases are activated in the epithelium of lenses exposed to thrombin. Conclusions. The results suggest the inhibitory effect of thrombin on lens active sodium-potassium transport could involve the activation of a receptor-second-messenger mechanism in intact lens cells. The response appears to involve a tyrosine kinase-mediated step. The functional significance of the thrombin-mediated change of lens active sodium-potassium transport is unclear since appreciable amounts of thrombin may only be presented to the lens during instances of blood-aqueous-barrier breakdown. It is possible that lens receptors are functionally activated by other proteases, possibly cathepsins, which may enter aqueous humor from the ciliary body.

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