Tibial dyschondroplasia-associated proteomic changes in chicken growth plate cartilage

K. S. Rasaputra, R. Liyanage, J. O. Lay, Fiona M McCarthy, N. C. Rath

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Tibial dyschondroplasia (TD) is a poultry leg problem that affects the proximal growth plate of the tibia, preventing its transition to bone. To understand the disease-induced proteomic changes, we compared the protein extracts of cartilage from normal and TD-affected growth plates. TD was induced by feeding thiram to chickens 2 wk before tissue harvest. Proteins were extracted from whole tissues and from conditioned media (CM) prepared by incubating appropriate growth plate tissues in serum-free culture medium for 48 hr. The extracts were prefractionated to contain proteins ranging between 10 and 100 kD. Equal amounts of proteins were subjected to 2D gel electrophoresis with three individual samples per group. The gels were silver stained, and digital images were compared and analyzed with Melanie software to determine differentially expressed protein spots. On comparison of two sets of gels, 47 matching spots were detected in tissue extracts and 27 in CM extracts. Among the matching spots, 12 were determined to be down-regulated in tissue extracts (P ≤ 0.05) and two in CM extracts (P ≤ 0.05) of TD-affected growth plates. Altogether, 32 protein spots could be identified in both tissue and CM extracts by in-gel trypsin digestion, followed by peptide mass fingerprinting and mass spectrometry (MS)/MS fragmentation. The down-regulated proteins included alpha-enolase, G protein, origin recognition complex, peptidyl prolyl isomerase, calumenin, type II collagen precursor, and the expressed sequence tag pgm2n.pk014.f20, a protein with homology to human reticulocalbin-3 (RCN3). Most of the down-regulated proteins are associated with signal transduction, energy metabolism, and secretory functions that are integral to cell viability. Consistent with our earlier findings that the TD chondrocytes are nonviable, the current results suggest that thiram very likely interferes with basic metabolic functions of chondrocytes, leading to their death and, consequently, to the pathogenesis of TD.

Original languageEnglish (US)
Pages (from-to)1166-1171
Number of pages6
JournalAvian Diseases
Volume54
Issue number4
DOIs
StatePublished - Dec 2010
Externally publishedYes

Fingerprint

Osteochondrodysplasias
dyschondroplasia
growth plate
Growth Plate
cartilage
Proteomics
proteomics
Cartilage
Chickens
chickens
Conditioned Culture Medium
extracts
Proteins
proteins
Thiram
thiram
Tissue Extracts
chondrocytes
Gels
gels

Keywords

  • chicken
  • growth plate
  • mass spectrometry
  • proteins
  • tibial dyschondroplasia

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Animals
  • Immunology and Microbiology(all)

Cite this

Tibial dyschondroplasia-associated proteomic changes in chicken growth plate cartilage. / Rasaputra, K. S.; Liyanage, R.; Lay, J. O.; McCarthy, Fiona M; Rath, N. C.

In: Avian Diseases, Vol. 54, No. 4, 12.2010, p. 1166-1171.

Research output: Contribution to journalArticle

Rasaputra, K. S. ; Liyanage, R. ; Lay, J. O. ; McCarthy, Fiona M ; Rath, N. C. / Tibial dyschondroplasia-associated proteomic changes in chicken growth plate cartilage. In: Avian Diseases. 2010 ; Vol. 54, No. 4. pp. 1166-1171.
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