Time course of ICAM-1 expression and leukocyte subset infiltration in rat forebrain ischemia

Wayne M. Clark, Jeffrey D. Lauten, Nikola Lessov, William Woodward, Bruce M Coull

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The time course of ICAM-1 expression and leukocyte subset infiltration was studied in a model of CNS reperfusion injury in adult rats. Leukocyte adhesion and infiltration, mediated in part by intercellular adhesion molecule-1 (ICAM-1), appears to potentiate CNS reperfusion injury. The timing and relationship between ICAM-1 staining and leukocyte infiltration postglobal CNS ischemia is unknown. Reversible forebrain ischemia was produced in 32 adult Sprague-Dawley rats using the two-vessel occlusion model with histologic analysis performed at specific intervals postischemia: 1, 3, 6, 12 and 24h, 4 and 7 d, or sham-operated controls (n=4 each group). Monoclonal antibodies against ICAM-1 (1A29 and TM8), a specific granulocyte (PMN) (HIS48), and a specific monocyte/macrophage (MØ)(ED1) were used. No specific leukocyte and only rare ICAM-1 vessel immunoreactivity was observed in sham controls. ICAM-1: Significant expression in microvessels beginning at 1 h with additional diffuse CA1 pyramidal layer staining beginning at 4 d. Leukocytes: NO PMN cells and rare MØ identified at 6 and 12 h. By 24 h: moderate infiltrate in areas of ICAM-1 expression of PMN and MØ. At 4 and 7 d: only MØ accumulation, cellular morphology now similar to microglia. The results of this study indicate that early and persistent ICAM-1 expression occurs following CNS ischemia with associated leukocyte infiltration.

Original languageEnglish (US)
Pages (from-to)213-230
Number of pages18
JournalMolecular and Chemical Neuropathology
Volume26
Issue number3
DOIs
StatePublished - Dec 1995
Externally publishedYes

Fingerprint

Intercellular Adhesion Molecule-1
Prosencephalon
Leukocytes
Ischemia
Reperfusion Injury
Staining and Labeling
Microglia
Microvessels
Granulocytes
Sprague Dawley Rats
Monocytes
Macrophages
Monoclonal Antibodies

Keywords

  • cerebral ischemia
  • ICAM-1
  • leukocytes
  • macrophage
  • microglia
  • reperfusion injury

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Molecular Biology

Cite this

Time course of ICAM-1 expression and leukocyte subset infiltration in rat forebrain ischemia. / Clark, Wayne M.; Lauten, Jeffrey D.; Lessov, Nikola; Woodward, William; Coull, Bruce M.

In: Molecular and Chemical Neuropathology, Vol. 26, No. 3, 12.1995, p. 213-230.

Research output: Contribution to journalArticle

Clark, Wayne M. ; Lauten, Jeffrey D. ; Lessov, Nikola ; Woodward, William ; Coull, Bruce M. / Time course of ICAM-1 expression and leukocyte subset infiltration in rat forebrain ischemia. In: Molecular and Chemical Neuropathology. 1995 ; Vol. 26, No. 3. pp. 213-230.
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