tinman-related genes expressed during heart development in Xenopus

Craig S. Newman, Paul A Krieg

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The tinman homeobox gene of Drosophila is absolutely required for development of the insect heart. This observation prompted the isolation of tinman-related genes from vertebrates, in the hope that the developmental function of the gene would be conserved between evolutionarily distinct species. The first vertebrate tinman gene, Nkx2-5, was isolated from mouse and subsequently, orthologues of Nkx2-5 have been isolated from a number of different species. In all cases, a conserved pattern of Nkx2-5 expression is observed in the developing heart, commencing prior to differentiation. Genetic oblation of Nkx2-5 in the mouse results in embryonic lethality due to heart defects, but most myocardial genes are expressed normally and a beating heart tube forms. This observation raises the possibility that additional genes related to Nkx2-5 are partially rescuing Nkx2-5 function in the null mouse. Recently, additional members of the tinman-related gene family have been discovered and characterized in a number of different species. Somewhat surprisingly, orthologous genes in different organisms can be rather divergent in sequence and may show completely different expression patterns. In at least some organisms, expression of the tinman-related genes is not observed in the heart. Due to the increasing number of family members and the somewhat divergent expression patterns, the precise role of the tinman- related genes in cardiac development remains on open question. In a search for additional tinman-related genes in the frog, Xenopus laevis, we have identified Nkx2-9, a novel member of the tinman-related gene family. Preliminary characterization reveals that Nkx2-9 is expressed in the cardiogenic region of the embryo prior to differentiation, but transcript levels decrease rapidly, in the heart, at about the time that differentiation commences.

Original languageEnglish (US)
Pages (from-to)230-238
Number of pages9
JournalDevelopmental Genetics
Volume22
Issue number3
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Xenopus
Genes
Vertebrates
Developmental Genes
Homeobox Genes
Xenopus laevis
Anura
Drosophila
Insects
Embryonic Structures

Keywords

  • Drosophila
  • Nkx genes
  • Tinman
  • Xenopus laevis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics

Cite this

tinman-related genes expressed during heart development in Xenopus. / Newman, Craig S.; Krieg, Paul A.

In: Developmental Genetics, Vol. 22, No. 3, 1998, p. 230-238.

Research output: Contribution to journalArticle

@article{418892a4447249cb876250452acce75d,
title = "tinman-related genes expressed during heart development in Xenopus",
abstract = "The tinman homeobox gene of Drosophila is absolutely required for development of the insect heart. This observation prompted the isolation of tinman-related genes from vertebrates, in the hope that the developmental function of the gene would be conserved between evolutionarily distinct species. The first vertebrate tinman gene, Nkx2-5, was isolated from mouse and subsequently, orthologues of Nkx2-5 have been isolated from a number of different species. In all cases, a conserved pattern of Nkx2-5 expression is observed in the developing heart, commencing prior to differentiation. Genetic oblation of Nkx2-5 in the mouse results in embryonic lethality due to heart defects, but most myocardial genes are expressed normally and a beating heart tube forms. This observation raises the possibility that additional genes related to Nkx2-5 are partially rescuing Nkx2-5 function in the null mouse. Recently, additional members of the tinman-related gene family have been discovered and characterized in a number of different species. Somewhat surprisingly, orthologous genes in different organisms can be rather divergent in sequence and may show completely different expression patterns. In at least some organisms, expression of the tinman-related genes is not observed in the heart. Due to the increasing number of family members and the somewhat divergent expression patterns, the precise role of the tinman- related genes in cardiac development remains on open question. In a search for additional tinman-related genes in the frog, Xenopus laevis, we have identified Nkx2-9, a novel member of the tinman-related gene family. Preliminary characterization reveals that Nkx2-9 is expressed in the cardiogenic region of the embryo prior to differentiation, but transcript levels decrease rapidly, in the heart, at about the time that differentiation commences.",
keywords = "Drosophila, Nkx genes, Tinman, Xenopus laevis",
author = "Newman, {Craig S.} and Krieg, {Paul A}",
year = "1998",
doi = "10.1002/(SICI)1520-6408(1998)22:3<230::AID-DVG5>3.0.CO;2-7",
language = "English (US)",
volume = "22",
pages = "230--238",
journal = "Genesis",
issn = "1526-954X",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - tinman-related genes expressed during heart development in Xenopus

AU - Newman, Craig S.

AU - Krieg, Paul A

PY - 1998

Y1 - 1998

N2 - The tinman homeobox gene of Drosophila is absolutely required for development of the insect heart. This observation prompted the isolation of tinman-related genes from vertebrates, in the hope that the developmental function of the gene would be conserved between evolutionarily distinct species. The first vertebrate tinman gene, Nkx2-5, was isolated from mouse and subsequently, orthologues of Nkx2-5 have been isolated from a number of different species. In all cases, a conserved pattern of Nkx2-5 expression is observed in the developing heart, commencing prior to differentiation. Genetic oblation of Nkx2-5 in the mouse results in embryonic lethality due to heart defects, but most myocardial genes are expressed normally and a beating heart tube forms. This observation raises the possibility that additional genes related to Nkx2-5 are partially rescuing Nkx2-5 function in the null mouse. Recently, additional members of the tinman-related gene family have been discovered and characterized in a number of different species. Somewhat surprisingly, orthologous genes in different organisms can be rather divergent in sequence and may show completely different expression patterns. In at least some organisms, expression of the tinman-related genes is not observed in the heart. Due to the increasing number of family members and the somewhat divergent expression patterns, the precise role of the tinman- related genes in cardiac development remains on open question. In a search for additional tinman-related genes in the frog, Xenopus laevis, we have identified Nkx2-9, a novel member of the tinman-related gene family. Preliminary characterization reveals that Nkx2-9 is expressed in the cardiogenic region of the embryo prior to differentiation, but transcript levels decrease rapidly, in the heart, at about the time that differentiation commences.

AB - The tinman homeobox gene of Drosophila is absolutely required for development of the insect heart. This observation prompted the isolation of tinman-related genes from vertebrates, in the hope that the developmental function of the gene would be conserved between evolutionarily distinct species. The first vertebrate tinman gene, Nkx2-5, was isolated from mouse and subsequently, orthologues of Nkx2-5 have been isolated from a number of different species. In all cases, a conserved pattern of Nkx2-5 expression is observed in the developing heart, commencing prior to differentiation. Genetic oblation of Nkx2-5 in the mouse results in embryonic lethality due to heart defects, but most myocardial genes are expressed normally and a beating heart tube forms. This observation raises the possibility that additional genes related to Nkx2-5 are partially rescuing Nkx2-5 function in the null mouse. Recently, additional members of the tinman-related gene family have been discovered and characterized in a number of different species. Somewhat surprisingly, orthologous genes in different organisms can be rather divergent in sequence and may show completely different expression patterns. In at least some organisms, expression of the tinman-related genes is not observed in the heart. Due to the increasing number of family members and the somewhat divergent expression patterns, the precise role of the tinman- related genes in cardiac development remains on open question. In a search for additional tinman-related genes in the frog, Xenopus laevis, we have identified Nkx2-9, a novel member of the tinman-related gene family. Preliminary characterization reveals that Nkx2-9 is expressed in the cardiogenic region of the embryo prior to differentiation, but transcript levels decrease rapidly, in the heart, at about the time that differentiation commences.

KW - Drosophila

KW - Nkx genes

KW - Tinman

KW - Xenopus laevis

UR - http://www.scopus.com/inward/record.url?scp=0031835362&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031835362&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1520-6408(1998)22:3<230::AID-DVG5>3.0.CO;2-7

DO - 10.1002/(SICI)1520-6408(1998)22:3<230::AID-DVG5>3.0.CO;2-7

M3 - Article

VL - 22

SP - 230

EP - 238

JO - Genesis

JF - Genesis

SN - 1526-954X

IS - 3

ER -