Tissue distribution, ontogeny and induction of the transporters Multidrug and toxin extrusion (MATE) 1 and MATE2 mRNA expression levels in mice

Andrew J. Lickteig, Xingguo Cheng, Lisa M. Augustine, Curtis D. Klaassen, Nathan J Cherrington

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Transporters are expressed in a wide variety of tissues where they perform the critical function of enabling anionic and cationic chemicals of exogenous and endogenous origin to cross otherwise impermeable cell membranes. The Multidrug and toxin extrusion (MATE) transporters mediate cellular efflux of a variety of organic cations, including many drugs. The purpose of the current study was to determine (1) constitutive expression levels of MATE mRNA in various tissues, (2) whether there are gender differences in the expression of MATEs, (3) the ontogenic expression pattern of MATE1 in kidney and (4) whether MATEs are pharmacologically inducible in liver via activation of known transcription factors. In both male and female mice, MATE1 mRNA levels were highest in the kidney, where male expression was higher than female. MATE2 mRNA expression levels were the highest in the testis, where high expression was localized to Sertoli cells, a critical cell type of the blood testis barrier. In female mice, MATE2 mRNA levels were expressed most highly in the colon. The ontogenic pattern of expression of MATE1 mRNA in the kidneys of both males and females was gradual, with levels increasing steadily from prenatal day - 2 to 45 days of age, and a gender difference appearing at day 30. Of the transcription factor activators examined (AhR, CAR, Nrf2, PPARα and PXR), none were capable of altering MATE1 or MATE2. The current findings support a potential role for MATE1 and MATE2 in a wide range of tissues and, notably, a unique role for MATE2 in the blood-testis barrier.

Original languageEnglish (US)
Pages (from-to)59-64
Number of pages6
JournalLife Sciences
Volume83
Issue number1-2
DOIs
StatePublished - Jul 4 2008

Fingerprint

Tissue Distribution
Extrusion
Tissue
Messenger RNA
Blood-Testis Barrier
Kidney
Blood
Transcription Factors
Peroxisome Proliferator-Activated Receptors
Sertoli Cells
Cell membranes
Liver
Cations
Testis
Colon
Chemical activation
Cell Membrane
Pharmaceutical Preparations

Keywords

  • MATE1
  • MATE2
  • Multidrug and toxin extrusion
  • Testis
  • Tissue distribution
  • Transporter

ASJC Scopus subject areas

  • Pharmacology

Cite this

Tissue distribution, ontogeny and induction of the transporters Multidrug and toxin extrusion (MATE) 1 and MATE2 mRNA expression levels in mice. / Lickteig, Andrew J.; Cheng, Xingguo; Augustine, Lisa M.; Klaassen, Curtis D.; Cherrington, Nathan J.

In: Life Sciences, Vol. 83, No. 1-2, 04.07.2008, p. 59-64.

Research output: Contribution to journalArticle

Lickteig, Andrew J. ; Cheng, Xingguo ; Augustine, Lisa M. ; Klaassen, Curtis D. ; Cherrington, Nathan J. / Tissue distribution, ontogeny and induction of the transporters Multidrug and toxin extrusion (MATE) 1 and MATE2 mRNA expression levels in mice. In: Life Sciences. 2008 ; Vol. 83, No. 1-2. pp. 59-64.
@article{c5a2362b9f1d4eab9f1d3702522110f1,
title = "Tissue distribution, ontogeny and induction of the transporters Multidrug and toxin extrusion (MATE) 1 and MATE2 mRNA expression levels in mice",
abstract = "Transporters are expressed in a wide variety of tissues where they perform the critical function of enabling anionic and cationic chemicals of exogenous and endogenous origin to cross otherwise impermeable cell membranes. The Multidrug and toxin extrusion (MATE) transporters mediate cellular efflux of a variety of organic cations, including many drugs. The purpose of the current study was to determine (1) constitutive expression levels of MATE mRNA in various tissues, (2) whether there are gender differences in the expression of MATEs, (3) the ontogenic expression pattern of MATE1 in kidney and (4) whether MATEs are pharmacologically inducible in liver via activation of known transcription factors. In both male and female mice, MATE1 mRNA levels were highest in the kidney, where male expression was higher than female. MATE2 mRNA expression levels were the highest in the testis, where high expression was localized to Sertoli cells, a critical cell type of the blood testis barrier. In female mice, MATE2 mRNA levels were expressed most highly in the colon. The ontogenic pattern of expression of MATE1 mRNA in the kidneys of both males and females was gradual, with levels increasing steadily from prenatal day - 2 to 45 days of age, and a gender difference appearing at day 30. Of the transcription factor activators examined (AhR, CAR, Nrf2, PPARα and PXR), none were capable of altering MATE1 or MATE2. The current findings support a potential role for MATE1 and MATE2 in a wide range of tissues and, notably, a unique role for MATE2 in the blood-testis barrier.",
keywords = "MATE1, MATE2, Multidrug and toxin extrusion, Testis, Tissue distribution, Transporter",
author = "Lickteig, {Andrew J.} and Xingguo Cheng and Augustine, {Lisa M.} and Klaassen, {Curtis D.} and Cherrington, {Nathan J}",
year = "2008",
month = "7",
day = "4",
doi = "10.1016/j.lfs.2008.05.004",
language = "English (US)",
volume = "83",
pages = "59--64",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "1-2",

}

TY - JOUR

T1 - Tissue distribution, ontogeny and induction of the transporters Multidrug and toxin extrusion (MATE) 1 and MATE2 mRNA expression levels in mice

AU - Lickteig, Andrew J.

AU - Cheng, Xingguo

AU - Augustine, Lisa M.

AU - Klaassen, Curtis D.

AU - Cherrington, Nathan J

PY - 2008/7/4

Y1 - 2008/7/4

N2 - Transporters are expressed in a wide variety of tissues where they perform the critical function of enabling anionic and cationic chemicals of exogenous and endogenous origin to cross otherwise impermeable cell membranes. The Multidrug and toxin extrusion (MATE) transporters mediate cellular efflux of a variety of organic cations, including many drugs. The purpose of the current study was to determine (1) constitutive expression levels of MATE mRNA in various tissues, (2) whether there are gender differences in the expression of MATEs, (3) the ontogenic expression pattern of MATE1 in kidney and (4) whether MATEs are pharmacologically inducible in liver via activation of known transcription factors. In both male and female mice, MATE1 mRNA levels were highest in the kidney, where male expression was higher than female. MATE2 mRNA expression levels were the highest in the testis, where high expression was localized to Sertoli cells, a critical cell type of the blood testis barrier. In female mice, MATE2 mRNA levels were expressed most highly in the colon. The ontogenic pattern of expression of MATE1 mRNA in the kidneys of both males and females was gradual, with levels increasing steadily from prenatal day - 2 to 45 days of age, and a gender difference appearing at day 30. Of the transcription factor activators examined (AhR, CAR, Nrf2, PPARα and PXR), none were capable of altering MATE1 or MATE2. The current findings support a potential role for MATE1 and MATE2 in a wide range of tissues and, notably, a unique role for MATE2 in the blood-testis barrier.

AB - Transporters are expressed in a wide variety of tissues where they perform the critical function of enabling anionic and cationic chemicals of exogenous and endogenous origin to cross otherwise impermeable cell membranes. The Multidrug and toxin extrusion (MATE) transporters mediate cellular efflux of a variety of organic cations, including many drugs. The purpose of the current study was to determine (1) constitutive expression levels of MATE mRNA in various tissues, (2) whether there are gender differences in the expression of MATEs, (3) the ontogenic expression pattern of MATE1 in kidney and (4) whether MATEs are pharmacologically inducible in liver via activation of known transcription factors. In both male and female mice, MATE1 mRNA levels were highest in the kidney, where male expression was higher than female. MATE2 mRNA expression levels were the highest in the testis, where high expression was localized to Sertoli cells, a critical cell type of the blood testis barrier. In female mice, MATE2 mRNA levels were expressed most highly in the colon. The ontogenic pattern of expression of MATE1 mRNA in the kidneys of both males and females was gradual, with levels increasing steadily from prenatal day - 2 to 45 days of age, and a gender difference appearing at day 30. Of the transcription factor activators examined (AhR, CAR, Nrf2, PPARα and PXR), none were capable of altering MATE1 or MATE2. The current findings support a potential role for MATE1 and MATE2 in a wide range of tissues and, notably, a unique role for MATE2 in the blood-testis barrier.

KW - MATE1

KW - MATE2

KW - Multidrug and toxin extrusion

KW - Testis

KW - Tissue distribution

KW - Transporter

UR - http://www.scopus.com/inward/record.url?scp=45249105082&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45249105082&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2008.05.004

DO - 10.1016/j.lfs.2008.05.004

M3 - Article

C2 - 18573474

AN - SCOPUS:45249105082

VL - 83

SP - 59

EP - 64

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 1-2

ER -