Tissue viability assessment via laser-based fluorescence monitor

Craig H. Curtis, Nasser N Peyghambarian, Eustace L. Dereniak, Edward Frink

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A preliminary study of the degree to which recently introduced inhalation anesthetics influence the intracellular energetic metabolism of isolated perfused rat livers is undertaken via NADH fluorometry. During liver transplantation, anesthesiologists desire to maintain a high level of metabolic energy status in newly transplanted liver tissue. Ischemic storage of donor liver tissue prior to transplantation is known to inhibit mitochondrial electron transfer, which results in decreased levels of ATP and increased levels of NADH in the stored tissue. The ability of transplanted liver tissue to regenerate ATP at normal levels is desirable for early post- operative recovery of liver function. Previous studies have examined the differential effects inhalation anesthetics have on the energetic metabolism of tissue at the cellular level; the trend of such agents is to induce a dose-dependent increase in NADH fluorescence in accordance with their strengths as general anesthetics. The present study evaluates the differential effects exhibited by new inhalation anesthetics on the return of function of energetic metabolism in liver tissue. The third-harmonic (355 nm) output of a Nd:YAG laser is spatially filtered and used as the excitation source for surface fluorometric measurements of isolated buffer-perfused rat livers. Lastly, maximum fluorescence emission versus spot-size are measured.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsRobert A. Lieberman, Halina Podbielska, Tuan Vo-Dinh
Pages258-262
Number of pages5
Volume2676
StatePublished - 1996
EventBiomedical Sensing, Imaging, and Tracking Technologies I - San Jose, CA, USA
Duration: Jan 29 1996Jan 31 1996

Other

OtherBiomedical Sensing, Imaging, and Tracking Technologies I
CitySan Jose, CA, USA
Period1/29/961/31/96

Fingerprint

viability
liver
Liver
monitors
Fluorescence
Tissue
anesthetics
Anesthetics
fluorescence
Lasers
lasers
respiration
metabolism
Metabolism
transplantation
adenosine triphosphate
Adenosinetriphosphate
rats
Rats
Surface measurement

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Curtis, C. H., Peyghambarian, N. N., Dereniak, E. L., & Frink, E. (1996). Tissue viability assessment via laser-based fluorescence monitor. In R. A. Lieberman, H. Podbielska, & T. Vo-Dinh (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2676, pp. 258-262)

Tissue viability assessment via laser-based fluorescence monitor. / Curtis, Craig H.; Peyghambarian, Nasser N; Dereniak, Eustace L.; Frink, Edward.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Robert A. Lieberman; Halina Podbielska; Tuan Vo-Dinh. Vol. 2676 1996. p. 258-262.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Curtis, CH, Peyghambarian, NN, Dereniak, EL & Frink, E 1996, Tissue viability assessment via laser-based fluorescence monitor. in RA Lieberman, H Podbielska & T Vo-Dinh (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 2676, pp. 258-262, Biomedical Sensing, Imaging, and Tracking Technologies I, San Jose, CA, USA, 1/29/96.
Curtis CH, Peyghambarian NN, Dereniak EL, Frink E. Tissue viability assessment via laser-based fluorescence monitor. In Lieberman RA, Podbielska H, Vo-Dinh T, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2676. 1996. p. 258-262
Curtis, Craig H. ; Peyghambarian, Nasser N ; Dereniak, Eustace L. ; Frink, Edward. / Tissue viability assessment via laser-based fluorescence monitor. Proceedings of SPIE - The International Society for Optical Engineering. editor / Robert A. Lieberman ; Halina Podbielska ; Tuan Vo-Dinh. Vol. 2676 1996. pp. 258-262
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