Tissue viability assessment via laser-based fluorescence monitor

Craig H. Curtis, Nasser Peyghambarian, Eustace L. Dereniak, Edward Frink

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A preliminary study of the degree to which recently introduced inhalation anesthetics influence the intracellular energetic metabolism of isolated perfused rat livers is undertaken via NADH fluorometry. During liver transplantation, anesthesiologists desire to maintain a high level of metabolic energy status in newly transplanted liver tissue. Ischemic storage of donor liver tissue prior to transplantation is known to inhibit mitochondrial electron transfer, which results in decreased levels of ATP and increased levels of NADH in the stored tissue. The ability of transplanted liver tissue to regenerate ATP at normal levels is desirable for early post- operative recovery of liver function. Previous studies have examined the differential effects inhalation anesthetics have on the energetic metabolism of tissue at the cellular level; the trend of such agents is to induce a dose-dependent increase in NADH fluorescence in accordance with their strengths as general anesthetics. The present study evaluates the differential effects exhibited by new inhalation anesthetics on the return of function of energetic metabolism in liver tissue. The third-harmonic (355 nm) output of a Nd:YAG laser is spatially filtered and used as the excitation source for surface fluorometric measurements of isolated buffer-perfused rat livers. Lastly, maximum fluorescence emission versus spot-size are measured.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsRobert A. Lieberman, Halina Podbielska, Tuan Vo-Dinh
Pages258-262
Number of pages5
StatePublished - Dec 1 1996
EventBiomedical Sensing, Imaging, and Tracking Technologies I - San Jose, CA, USA
Duration: Jan 29 1996Jan 31 1996

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2676
ISSN (Print)0277-786X

Other

OtherBiomedical Sensing, Imaging, and Tracking Technologies I
CitySan Jose, CA, USA
Period1/29/961/31/96

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Tissue viability assessment via laser-based fluorescence monitor'. Together they form a unique fingerprint.

  • Cite this

    Curtis, C. H., Peyghambarian, N., Dereniak, E. L., & Frink, E. (1996). Tissue viability assessment via laser-based fluorescence monitor. In R. A. Lieberman, H. Podbielska, & T. Vo-Dinh (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (pp. 258-262). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2676).