Topography and numerical densities of intramembrane particles in chemical carcinogen-induced urinary bladder carcinomas in Fischer rats

B. U. Pauli, G. H. Friedell, Ronald S Weinstein

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Integral membrane proteins are visualized as intramembrane particles (IMP) at the cleaved surfaces of freeze-fractured plasma membranes. Topographical distribution of the IMP of urinary bladder epithelial cell membranes in normal Fischer rat bladder and noninvasive and invasive N-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide (FANFT)-induced bladder tumors are shown to be significantly different. Using several statistical methods that test IMP topography vis-a-vis the Poisson (random) hypothesis, it is demonstrated that IMP are mathematically randomly distributed in the large majority of plasma membranes of cells in normal rat bladder epithelium and in invasive N-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide tumors. In noninvasive rat bladder carcinomas, IMP are in a lattice-like arrangement in half of the tumor cells and randomly distributed in the remainder. IMP numerical densities are also altered in the course of neoplastic transformation. IMP are equally increased above control values in both noninvasive and invasive N-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide tumors. Although transformation into noninvasive tumors is associated with increased numbers of IMP, there is no evidence that this parameter is specifically related to tumor biologic behavior in this model system.

Original languageEnglish (US)
Pages (from-to)565-573
Number of pages9
JournalLaboratory Investigation
Volume39
Issue number6
StatePublished - 1978
Externally publishedYes

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Inbred F344 Rats
Carcinogens
Urinary Bladder
Carcinoma
Cell Membrane
Neoplasms
FANFT
Plasma Cells
Urinary Bladder Neoplasms
Membrane Proteins
Epithelium
Epithelial Cells
formamide

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Topography and numerical densities of intramembrane particles in chemical carcinogen-induced urinary bladder carcinomas in Fischer rats. / Pauli, B. U.; Friedell, G. H.; Weinstein, Ronald S.

In: Laboratory Investigation, Vol. 39, No. 6, 1978, p. 565-573.

Research output: Contribution to journalArticle

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