Towards normalization of soybean somatic embryo maturation

Monica Schmidt, D. M. Tucker, E. B. Cahoon, W. A. Parrott

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Soybean (Glycine max L. Merrill) somatic embryos have been useful for assaying seed-specific traits prior to plant recovery. Such traits could be assessed more accurately if somatic embryos more closely mimicked seed development. Amino acid supplements, carbon source, and abscisic acid and basal salt formulations were tested in an effort to modify existing soybean embryogenesis histodifferentiation/maturation media to further normalize the development of soybean somatic embryos. The resultant liquid medium, referred to as soybean histodifferentiation and maturation medium (SHaM), consists of FNL basal salts, 3% sucrose, 3% sorbitol, filter-sterilized 30 mM glutamine and 1 mM methionine. SHaM-derived somatic embryos are more similar to seed in terms of protein and fatty acid/lipid composition, and conversion ability, than somatic embryos obtained from traditional soybean histodifferentiation and maturation media.

Original languageEnglish (US)
Pages (from-to)383-391
Number of pages9
JournalPlant Cell Reports
Volume24
Issue number7
DOIs
StatePublished - Sep 2005
Externally publishedYes

Fingerprint

somatic embryos
soybeans
salts
lipid composition
sorbitol
seeds
seed development
glutamine
abscisic acid
methionine
Glycine max
embryogenesis
sucrose
fatty acids
amino acids
liquids
carbon
proteins

Keywords

  • Glutamine
  • Glycine max
  • Methionine
  • Oil
  • Somatic embryogenesis

ASJC Scopus subject areas

  • Plant Science

Cite this

Towards normalization of soybean somatic embryo maturation. / Schmidt, Monica; Tucker, D. M.; Cahoon, E. B.; Parrott, W. A.

In: Plant Cell Reports, Vol. 24, No. 7, 09.2005, p. 383-391.

Research output: Contribution to journalArticle

Schmidt, Monica ; Tucker, D. M. ; Cahoon, E. B. ; Parrott, W. A. / Towards normalization of soybean somatic embryo maturation. In: Plant Cell Reports. 2005 ; Vol. 24, No. 7. pp. 383-391.
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