Toxicity of cisplatin and mercuric chloride in human kidney cortical slices

R. L. Fisher, J. T. Sannik, A Jay Gandolfi, K. Brendel

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

1 - Organ specific toxicity such as nephrotoxicity is often investigated with the use of in vivo or in vitro animal models. 2 - It would be beneficial if these findings could be verified in a human in vitro system which utilizes non-transplantable human kidneys. 3 - Non-transplantable human kidneys were decapsulated, cut in half along the long axis, cores made perpendicular to the hemisphere precision-cut renal cortical slices produced. 4 - These human kidney slices were incubated for 6, 12, 18 and 24 h, viability assessed using intracellular K+ content, protein synthesis and organic ion transport and the potential nephrotoxicity of cisplatin (0.25, 0.5 and 1.0 mM) and mercuric chloride (10, 50 and 100 μM) on these slices were examined. 5 - Control human kidney slices were viable for up to 24 h using all viability parameters while a dose- and time-dependent toxic response was seen using both cisplatin and mercuric chloride. 6 - Cisplatin was more nephrotoxic in this human in vitro system than in previously investigated in vitro animal systems whereas mercuric chloride was similar in both systems. 7 - These results indicate that human renal cortical slices are useful in predicting and verifying potentially nephrotoxic compounds in man.

Original languageEnglish (US)
Pages (from-to)517-523
Number of pages7
JournalHuman and Experimental Toxicology
Volume13
Issue number8
StatePublished - 1994

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Mercuric Chloride
Cisplatin
Toxicity
Kidney
Animals
Poisons
Ions
Synthetic Chemistry Techniques
Ion Transport
Animal Models
In Vitro Techniques

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Toxicity of cisplatin and mercuric chloride in human kidney cortical slices. / Fisher, R. L.; Sannik, J. T.; Gandolfi, A Jay; Brendel, K.

In: Human and Experimental Toxicology, Vol. 13, No. 8, 1994, p. 517-523.

Research output: Contribution to journalArticle

Fisher, R. L. ; Sannik, J. T. ; Gandolfi, A Jay ; Brendel, K. / Toxicity of cisplatin and mercuric chloride in human kidney cortical slices. In: Human and Experimental Toxicology. 1994 ; Vol. 13, No. 8. pp. 517-523.
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