Toxicity of halothane in guinea pig liver slices

H. N. Ghantous, J. Fernando, A Jay Gandolfi, K. Brendel

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Guinea pigs have proven to be a reliable model of halothane associated hepatotoxicity. An in vitro system with Hartley male guinea pig liver tissue was designed to assess the toxicity of halothane and other volatile anesthetics in the target organ. Precision-cut guinea pig liver slices (250-300 μm) were incubated in sealed roller vials containing Krebs-Henseleit buffer (plus vitamins, amino acids, glutamine, gentamycin) at 37°C, under 95%, 21% and 5% O2/CO2 atmospheres. Halothane (10-15 μl) was injected through a Teflon septa cap on a filter paper wick and vaporized. Viability of the slices was monitored by measuring intracellular K+ content which was maintained under 95% O2 up to 24 h. A dose-and time-related decrease in intracellular slice K+ by 1.9, 2.1, 2.7 mM halothane in the media was observed. At 2.7 mM halothane a direct physio-chemical effect may be occurring since incubating liver slices from allylisopropyl-acetamide-treated animals did not protect against the drop in intracellular K+. Concentration/time responses of halothane, d-halothane, enflurane, isoflurane and sevoflurane were compared. Sevoflurane had no effect on the liver slice K+ content up to 24 h while the other anesthetics caused the following rank-order decrease in intracellular K+ content: halothane > isoflurane and enflurane > d-halothane. Precision-cut cultured guinea pig liver slices offer a system where the target tissue for intoxication by anesthetics can be examined for its susceptibility and mechanism of intoxication.

Original languageEnglish (US)
Pages (from-to)59-69
Number of pages11
JournalToxicology
Volume62
Issue number1
DOIs
StatePublished - May 14 1990

Fingerprint

Halothane
Liver
Toxicity
Guinea Pigs
Anesthetics
Enflurane
Isoflurane
Capillary Action
Tissue
Polytetrafluoroethylene
Gentamicins
Glutamine
Atmosphere
Vitamins
Animals
Amino Acids

Keywords

  • d-Halothane
  • Enflurane Sevoflurane
  • Halothane
  • In Vitro
  • Isoflurane
  • Liver
  • Slices
  • Toxicity

ASJC Scopus subject areas

  • Toxicology

Cite this

Toxicity of halothane in guinea pig liver slices. / Ghantous, H. N.; Fernando, J.; Gandolfi, A Jay; Brendel, K.

In: Toxicology, Vol. 62, No. 1, 14.05.1990, p. 59-69.

Research output: Contribution to journalArticle

Ghantous, HN, Fernando, J, Gandolfi, AJ & Brendel, K 1990, 'Toxicity of halothane in guinea pig liver slices', Toxicology, vol. 62, no. 1, pp. 59-69. https://doi.org/10.1016/0300-483X(90)90031-B
Ghantous, H. N. ; Fernando, J. ; Gandolfi, A Jay ; Brendel, K. / Toxicity of halothane in guinea pig liver slices. In: Toxicology. 1990 ; Vol. 62, No. 1. pp. 59-69.
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