Toxoplasma co-opts host cells it does not invade

Anita A Koshy, Hans K. Dietrich, David A. Christian, Jason H. Melehani, Anjali J. Shastri, Christopher A. Hunter, John C. Boothroyd

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Like many intracellular microbes, the protozoan parasite Toxoplasma gondii injects effector proteins into cells it invades. One group of these effector proteins is injected from specialized organelles called the rhoptries, which have previously been described to discharge their contents only during successful invasion of a host cell. In this report, using several reporter systems, we show that in vitro the parasite injects rhoptry proteins into cells it does not productively invade and that the rhoptry effector proteins can manipulate the uninfected cell in a similar manner to infected cells. In addition, as one of the reporter systems uses a rhoptry:Cre recombinase fusion protein, we show that in Cre-reporter mice infected with an encysting Toxoplasma-Cre strain, uninfected-injected cells, which could be derived from aborted invasion or cell-intrinsic killing after invasion, are actually more common than infected-injected cells, especially in the mouse brain, where Toxoplasma encysts and persists. This phenomenon has important implications for how Toxoplasma globally affects its host and opens a new avenue for how other intracellular microbes may similarly manipulate the host environment at large.

Original languageEnglish (US)
Article numbere1002825
Pages (from-to)18
Number of pages1
JournalPLoS Pathogens
Volume8
Issue number7
DOIs
StatePublished - Jul 2012
Externally publishedYes

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Toxoplasma
Proteins
Parasites
Organelles
Brain

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Koshy, A. A., Dietrich, H. K., Christian, D. A., Melehani, J. H., Shastri, A. J., Hunter, C. A., & Boothroyd, J. C. (2012). Toxoplasma co-opts host cells it does not invade. PLoS Pathogens, 8(7), 18. [e1002825]. https://doi.org/10.1371/journal.ppat.1002825

Toxoplasma co-opts host cells it does not invade. / Koshy, Anita A; Dietrich, Hans K.; Christian, David A.; Melehani, Jason H.; Shastri, Anjali J.; Hunter, Christopher A.; Boothroyd, John C.

In: PLoS Pathogens, Vol. 8, No. 7, e1002825, 07.2012, p. 18.

Research output: Contribution to journalArticle

Koshy, AA, Dietrich, HK, Christian, DA, Melehani, JH, Shastri, AJ, Hunter, CA & Boothroyd, JC 2012, 'Toxoplasma co-opts host cells it does not invade', PLoS Pathogens, vol. 8, no. 7, e1002825, pp. 18. https://doi.org/10.1371/journal.ppat.1002825
Koshy AA, Dietrich HK, Christian DA, Melehani JH, Shastri AJ, Hunter CA et al. Toxoplasma co-opts host cells it does not invade. PLoS Pathogens. 2012 Jul;8(7):18. e1002825. https://doi.org/10.1371/journal.ppat.1002825
Koshy, Anita A ; Dietrich, Hans K. ; Christian, David A. ; Melehani, Jason H. ; Shastri, Anjali J. ; Hunter, Christopher A. ; Boothroyd, John C. / Toxoplasma co-opts host cells it does not invade. In: PLoS Pathogens. 2012 ; Vol. 8, No. 7. pp. 18.
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