Toxoplasma gondii: Fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts

Keith A Joiner, S. A. Fuhrman, H. M. Miettinen, L. H. Kasper, I. Mellman

Research output: Contribution to journalArticle

360 Citations (Scopus)

Abstract

After actively entering its host cells, the protozoan parasite Toxoplasma gondii resides in an intracellular vacuole that is completely unable to fuse with other endocytic or biosynthetic organelles. The fusion blocking requires entry of viable organisms but is irreversible: fusion competence of the vacuole is not restored if the parasite is killed after entry. The fusion block can be overcome, however, by altering the parasite's route of entry. Thus, phagocytosis of viable antibody-coated T. gondii by Chinese hamster ovary cells transfected with macrophage-lymphocyte Fc receptors results in the formation of vacuoles that are capable of both fusion and acidification. Phagocytosis and fusion appear to involve a domain of the Fc receptor cytoplasmic tail distinct from that required for localization at clathrin-coated pits. These results suggest that the mechanism of fusion inhibition is likely to reflect a modification of the vacuole membrane at the time of its formation, as opposed to the secretion of a soluble inhibitor by the parasite.

Original languageEnglish (US)
Pages (from-to)641-646
Number of pages6
JournalScience
Volume249
Issue number4969
StatePublished - Aug 10 1990
Externally publishedYes

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Fc Receptors
Toxoplasma
Vacuoles
Mental Competency
Parasites
Fibroblasts
Phagocytosis
Clathrin
Cricetulus
Organelles
Tail
Ovary
Macrophages
Lymphocytes
Membranes
Antibodies

ASJC Scopus subject areas

  • General

Cite this

Joiner, K. A., Fuhrman, S. A., Miettinen, H. M., Kasper, L. H., & Mellman, I. (1990). Toxoplasma gondii: Fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts. Science, 249(4969), 641-646.

Toxoplasma gondii : Fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts. / Joiner, Keith A; Fuhrman, S. A.; Miettinen, H. M.; Kasper, L. H.; Mellman, I.

In: Science, Vol. 249, No. 4969, 10.08.1990, p. 641-646.

Research output: Contribution to journalArticle

Joiner, KA, Fuhrman, SA, Miettinen, HM, Kasper, LH & Mellman, I 1990, 'Toxoplasma gondii: Fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts', Science, vol. 249, no. 4969, pp. 641-646.
Joiner KA, Fuhrman SA, Miettinen HM, Kasper LH, Mellman I. Toxoplasma gondii: Fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts. Science. 1990 Aug 10;249(4969):641-646.
Joiner, Keith A ; Fuhrman, S. A. ; Miettinen, H. M. ; Kasper, L. H. ; Mellman, I. / Toxoplasma gondii : Fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts. In: Science. 1990 ; Vol. 249, No. 4969. pp. 641-646.
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