Toxoplasma gondii: Fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts

K. A. Joiner, S. A. Fuhrman, H. M. Miettinen, L. H. Kasper, I. Mellman

Research output: Contribution to journalArticle

360 Scopus citations

Abstract

After actively entering its host cells, the protozoan parasite Toxoplasma gondii resides in an intracellular vacuole that is completely unable to fuse with other endocytic or biosynthetic organelles. The fusion blocking requires entry of viable organisms but is irreversible: fusion competence of the vacuole is not restored if the parasite is killed after entry. The fusion block can be overcome, however, by altering the parasite's route of entry. Thus, phagocytosis of viable antibody-coated T. gondii by Chinese hamster ovary cells transfected with macrophage-lymphocyte Fc receptors results in the formation of vacuoles that are capable of both fusion and acidification. Phagocytosis and fusion appear to involve a domain of the Fc receptor cytoplasmic tail distinct from that required for localization at clathrin-coated pits. These results suggest that the mechanism of fusion inhibition is likely to reflect a modification of the vacuole membrane at the time of its formation, as opposed to the secretion of a soluble inhibitor by the parasite.

Original languageEnglish (US)
Pages (from-to)641-646
Number of pages6
JournalScience
Volume249
Issue number4969
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Toxoplasma gondii: Fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts'. Together they form a unique fingerprint.

  • Cite this