Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions

Anita A. Koshy, Ashley E. Fouts, Melissa B. Lodoen, Ozan Alkan, Helen M. Blau, John C. Boothroyd

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

We describe a Toxoplasma gondii strain that will permit the use of site-specific recombination to study the host-parasite interactions of this organism. This Toxoplasma strain efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in 95% of infected host cells. By infecting Cre-reporter mice with these parasites, we also monitored host-cell infection in vivo.

Original languageEnglish (US)
Pages (from-to)307-309
Number of pages3
JournalNature Methods
Volume7
Issue number4
DOIs
StatePublished - Apr 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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