Transcriptional analyses of steroid-regulated gene networks

Margaret M Briehl, Francis A. Flomerfelt, Xi Ping Wu, Roger Miesfeld

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

It has been proposed that cell-specific responses to steroid action are the result of coordinate expression of steroid gene networks. Using three different cell systems, we have performed transcriptional analyses to determine if the observed hormone-induced alterations in gene expression are consist-ent with a limited number of potential target genes in any one cell type. Our results indicate that greater than 95% of the transcripts in dexamethasonetreated rat hepatoma (HTC), or mouse lymphoma (WEHI7) cells, are similar to the mRNAs in untreated cells based on subtraction hybridization. In addition, we find that although the castration-induced expression of androgen-regulated transcripts in the rat ventral prostate (RVP) is significantly different between normal and castrated rats (19%), the majority of these mRNAs are accounted for by the over abundance of sulfated glycoprotein-2 sequences. Specifically, analysis of an RVP subtracted cDNA library revealed that sulfated glycoprotein-2 mRNA masked the presence of less abundant differentially expressed sequences, confirming that the actual size of the RVP androgen gene network is small. We conclude that steroid-mediated changes in transcription accurately reflect the expression of a few cell-specific target genes, and thus support the model of steroid gene networks. The potential to characterize key elements which determine both the time course and magnitude of cell-specific hormone responses is discussed.

Original languageEnglish (US)
Pages (from-to)287-294
Number of pages8
JournalMolecular Endocrinology
Volume4
Issue number2
StatePublished - Feb 1990

Fingerprint

Gene Regulatory Networks
Steroids
Clusterin
Prostate
Androgens
Stored Messenger RNA
Hormones
Messenger RNA
Castration
Gene Library
Genes
Hepatocellular Carcinoma
Lymphoma
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Transcriptional analyses of steroid-regulated gene networks. / Briehl, Margaret M; Flomerfelt, Francis A.; Wu, Xi Ping; Miesfeld, Roger.

In: Molecular Endocrinology, Vol. 4, No. 2, 02.1990, p. 287-294.

Research output: Contribution to journalArticle

Briehl, Margaret M ; Flomerfelt, Francis A. ; Wu, Xi Ping ; Miesfeld, Roger. / Transcriptional analyses of steroid-regulated gene networks. In: Molecular Endocrinology. 1990 ; Vol. 4, No. 2. pp. 287-294.
@article{83e97b2a6ccb43c8b2f3145650e2e705,
title = "Transcriptional analyses of steroid-regulated gene networks",
abstract = "It has been proposed that cell-specific responses to steroid action are the result of coordinate expression of steroid gene networks. Using three different cell systems, we have performed transcriptional analyses to determine if the observed hormone-induced alterations in gene expression are consist-ent with a limited number of potential target genes in any one cell type. Our results indicate that greater than 95{\%} of the transcripts in dexamethasonetreated rat hepatoma (HTC), or mouse lymphoma (WEHI7) cells, are similar to the mRNAs in untreated cells based on subtraction hybridization. In addition, we find that although the castration-induced expression of androgen-regulated transcripts in the rat ventral prostate (RVP) is significantly different between normal and castrated rats (19{\%}), the majority of these mRNAs are accounted for by the over abundance of sulfated glycoprotein-2 sequences. Specifically, analysis of an RVP subtracted cDNA library revealed that sulfated glycoprotein-2 mRNA masked the presence of less abundant differentially expressed sequences, confirming that the actual size of the RVP androgen gene network is small. We conclude that steroid-mediated changes in transcription accurately reflect the expression of a few cell-specific target genes, and thus support the model of steroid gene networks. The potential to characterize key elements which determine both the time course and magnitude of cell-specific hormone responses is discussed.",
author = "Briehl, {Margaret M} and Flomerfelt, {Francis A.} and Wu, {Xi Ping} and Roger Miesfeld",
year = "1990",
month = "2",
language = "English (US)",
volume = "4",
pages = "287--294",
journal = "Molecular Endocrinology",
issn = "0888-8809",
publisher = "The Endocrine Society",
number = "2",

}

TY - JOUR

T1 - Transcriptional analyses of steroid-regulated gene networks

AU - Briehl, Margaret M

AU - Flomerfelt, Francis A.

AU - Wu, Xi Ping

AU - Miesfeld, Roger

PY - 1990/2

Y1 - 1990/2

N2 - It has been proposed that cell-specific responses to steroid action are the result of coordinate expression of steroid gene networks. Using three different cell systems, we have performed transcriptional analyses to determine if the observed hormone-induced alterations in gene expression are consist-ent with a limited number of potential target genes in any one cell type. Our results indicate that greater than 95% of the transcripts in dexamethasonetreated rat hepatoma (HTC), or mouse lymphoma (WEHI7) cells, are similar to the mRNAs in untreated cells based on subtraction hybridization. In addition, we find that although the castration-induced expression of androgen-regulated transcripts in the rat ventral prostate (RVP) is significantly different between normal and castrated rats (19%), the majority of these mRNAs are accounted for by the over abundance of sulfated glycoprotein-2 sequences. Specifically, analysis of an RVP subtracted cDNA library revealed that sulfated glycoprotein-2 mRNA masked the presence of less abundant differentially expressed sequences, confirming that the actual size of the RVP androgen gene network is small. We conclude that steroid-mediated changes in transcription accurately reflect the expression of a few cell-specific target genes, and thus support the model of steroid gene networks. The potential to characterize key elements which determine both the time course and magnitude of cell-specific hormone responses is discussed.

AB - It has been proposed that cell-specific responses to steroid action are the result of coordinate expression of steroid gene networks. Using three different cell systems, we have performed transcriptional analyses to determine if the observed hormone-induced alterations in gene expression are consist-ent with a limited number of potential target genes in any one cell type. Our results indicate that greater than 95% of the transcripts in dexamethasonetreated rat hepatoma (HTC), or mouse lymphoma (WEHI7) cells, are similar to the mRNAs in untreated cells based on subtraction hybridization. In addition, we find that although the castration-induced expression of androgen-regulated transcripts in the rat ventral prostate (RVP) is significantly different between normal and castrated rats (19%), the majority of these mRNAs are accounted for by the over abundance of sulfated glycoprotein-2 sequences. Specifically, analysis of an RVP subtracted cDNA library revealed that sulfated glycoprotein-2 mRNA masked the presence of less abundant differentially expressed sequences, confirming that the actual size of the RVP androgen gene network is small. We conclude that steroid-mediated changes in transcription accurately reflect the expression of a few cell-specific target genes, and thus support the model of steroid gene networks. The potential to characterize key elements which determine both the time course and magnitude of cell-specific hormone responses is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0025270579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025270579&partnerID=8YFLogxK

M3 - Article

VL - 4

SP - 287

EP - 294

JO - Molecular Endocrinology

JF - Molecular Endocrinology

SN - 0888-8809

IS - 2

ER -