Transcriptional analysis of Penaeus stylirostris densovirus genes

Arun K. Dhar, Krista N. Kaizer, Dilip K. Lakshman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Penaeus stylirostris densovirus (PstDNV) genome contains three open reading frames (ORFs), left, middle, and right, which encode a non-structural (NS) protein, an unknown protein, and a capsid protein (CP), respectively. Transcription mapping revealed that P2, P11 and P61 promoters transcribe the left, middle and right ORFs. NS transcript uses the D1/A1 donor/acceptor sites for splicing and has two alternate transcription termination sites (TTS) that were different from the previously predicted TTS. The transcription initiation site (TIS) and the TTS for the middle and the right ORFs conform to predicted sites. PstDNV transcript quantification in infected shrimp revealed that the NS and CP transcripts were expressed at an equivalent level and significantly higher than the middle ORF transcript. In vitro assay showed that P2 had the highest promoter activity followed by P11 and P61. Transcription mapping data provided new insights into PstDNV gene expression strategy.

Original languageEnglish (US)
Pages (from-to)112-120
Number of pages9
JournalVirology
Volume402
Issue number1
DOIs
StatePublished - Jun 1 2010
Externally publishedYes

Fingerprint

Densovirinae
Densovirus
Molecular Sequence Data
Genetic Transcription
Penaeidae
Sequence Alignment
Transcription Initiation Site
Gene Expression Profiling
Genetic Promoter Regions
Open Reading Frames
Capsid Proteins
Genes
Proteins
Genome
Gene Expression

Keywords

  • IHHNV
  • Infectious hypodermal and hematopoietic Necrosis virus
  • Penaeus stylirostris densovirus
  • PstDNV
  • Shrimp

ASJC Scopus subject areas

  • Virology

Cite this

Transcriptional analysis of Penaeus stylirostris densovirus genes. / Dhar, Arun K.; Kaizer, Krista N.; Lakshman, Dilip K.

In: Virology, Vol. 402, No. 1, 01.06.2010, p. 112-120.

Research output: Contribution to journalArticle

Dhar, Arun K. ; Kaizer, Krista N. ; Lakshman, Dilip K. / Transcriptional analysis of Penaeus stylirostris densovirus genes. In: Virology. 2010 ; Vol. 402, No. 1. pp. 112-120.
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