Transcriptional and pathway analysis in the hypothalamus of newly hatched chicks during fasting and delayed feeding

Stacy E. Higgins, Laura E. Ellestad, Nares Trakooljul, Fiona M McCarthy, Jason Saliba, Larry A. Cogburn, Tom E. Porter

Research output: Contribution to journalArticle

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Abstract

Background: The hypothalamus plays a central role in regulating appetite and metabolism. However, the gene networks within the hypothalamus that regulate feed intake and metabolism, and the effects of fasting on those pathways are not completely understood in any species. The present experiment evaluated global hypothalamic gene expression in newly hatched chicks using microarray analysis to elucidate genes and pathways regulated by feeding, fasting, and delayed feeding. Ten groups of chicks were sampled over four days post-hatch, including fed, fasted, and 48 h fasted followed by access to feed for 4 h, 24 h, and 48 h. Hypothalamic samples were collected for microarray analysis (n = 4). Expression patterns of selected genes were confirmed by quantitative real-time PCR. Pathway analysis of the microarray results predicted a network of genes involved in neuropeptide or neurotransmitter signaling. To confirm the functionality of this predicted gene network, hypothalamic neurons from fed and fasted chicks were isolated and cultured in the presence of neuropeptide Y, somatostatin, α-melanocyte stimulating hormone, norepinephrine, and L-phospho-serine. Results confirmed functional relationships among members of the predicted gene network. Moreover, the effects observed were dependant upon the nutritional state of the animals (fed vs. fasted).Results: Differences in gene expression (≥ 1.6 fold) were detected in 1,272 genes between treatments, and of those, 119 genes were significantly (P < 0.05) different. Pathway Miner analysis revealed that six genes (SSTR5, NPY5R, POMC, ADRB2, GRM8, and RLN3) were associated within a gene network. In vitro experiments with primary hypothalamic neurons confirmed that receptor agonists involved in this network regulated expression of other genes in the predicted network, and this regulation within the network was influenced by the nutritional status and age of the chick.Conclusions: Microarray analysis of the hypothalamus during different nutritional states revealed that many genes are differentially regulated. We found that functional interactions exist among six differentially regulated genes associated within a putative gene network from this experiment. Considering that POMC, an important gene in controlling metabolism, was central to this network, this gene network may play an important role in regulation of feeding and metabolism in birds.

Original languageEnglish (US)
Article number162
JournalBMC Genomics
Volume11
Issue number1
DOIs
StatePublished - Mar 9 2010
Externally publishedYes

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Gene Regulatory Networks
Hypothalamus
Fasting
Microarray Analysis
Genes
Pro-Opiomelanocortin
Gene Expression
Melanocyte-Stimulating Hormones
Neurons
Neuropeptide Y
Appetite
Somatostatin
Neuropeptides
Nutritional Status
Serine
Birds
Neurotransmitter Agents
Real-Time Polymerase Chain Reaction
Norepinephrine

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Transcriptional and pathway analysis in the hypothalamus of newly hatched chicks during fasting and delayed feeding. / Higgins, Stacy E.; Ellestad, Laura E.; Trakooljul, Nares; McCarthy, Fiona M; Saliba, Jason; Cogburn, Larry A.; Porter, Tom E.

In: BMC Genomics, Vol. 11, No. 1, 162, 09.03.2010.

Research output: Contribution to journalArticle

Higgins, Stacy E. ; Ellestad, Laura E. ; Trakooljul, Nares ; McCarthy, Fiona M ; Saliba, Jason ; Cogburn, Larry A. ; Porter, Tom E. / Transcriptional and pathway analysis in the hypothalamus of newly hatched chicks during fasting and delayed feeding. In: BMC Genomics. 2010 ; Vol. 11, No. 1.
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AU - Higgins, Stacy E.

AU - Ellestad, Laura E.

AU - Trakooljul, Nares

AU - McCarthy, Fiona M

AU - Saliba, Jason

AU - Cogburn, Larry A.

AU - Porter, Tom E.

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