Transcriptional control is relevant in the modulation of mosquito ferritin synthesis by iron

Daphne Q.D. Pham, Joy J. Winzerling, Mark S. Dodson, John H. Law

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

In yellow fever mosquito cells (Aag2 clone), iron treatment induces a threefold increase in ferritin message (fer mRNA) and protein (ferritin) by 16 h. These data contrast with work in mammalian hepatocytes and fibroblasts in which fer mRNA levels do not change with iron stimulation, but ferritin levels increase 50-fold. Pretreatment of the Aag2 cells with actinomycin D blocks induction offer mRNA and reduces the ferritin subunit synthesis, suggesting that iron induction of ferritin subunit synthesis is subjected to transcriptional control. A putative iron-regulatory protein has also been identified in cytoplasmic extracts from Aag2 cells.

Original languageEnglish (US)
Pages (from-to)236-240
Number of pages5
JournalEuropean Journal of Biochemistry
Volume266
Issue number1
DOIs
StatePublished - Nov 15 1999

Keywords

  • Aedes aegypti
  • Ferritin
  • Mosquito
  • Transcription
  • Translation

ASJC Scopus subject areas

  • Biochemistry

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