Transcriptional networks specifying homeostatic and inflammatory programs of gene expression in human aortic endothelial cells

Nicholas T. Hogan, Michael B. Whalen, Lindsey K. Stolze, Nizar K. Hadeli, Michael T. Lam, James R. Springstead, Christopher K. Glass, Casey E. Romanoski

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Endothelial cells (ECs) are critical determinants of vascular homeostasis and inflammation, but transcriptional mechanisms specifying their identities and functional states remain poorly understood. Here, we report a genome-wide assessment of regulatory landscapes of primary human aortic endothelial cells (HAECs) under basal and activated conditions, enabling inference of transcription factor networks that direct homeostatic and pro-inflammatory programs. We demonstrate that 43% of detected enhancers are EC-specific and contain SNPs associated to cardiovascular disease and hypertension. We provide evidence that AP1, ETS, and GATA transcription factors play key roles in HAEC transcription by co-binding enhancers associated with EC-specific genes. We further demonstrate that exposure of HAECs to oxidized phospholipids or pro-inflammatory cytokines results in signal-specific alterations in enhancer landscapes and associate with coordinated binding of CEBPD, IRF1, and NFkB. Collectively, these findings identify cis-regulatory elements and corresponding trans-acting factors that contribute to EC identity and their specific responses to pro-inflammatory stimuli.

Original languageEnglish (US)
Article numbere22536
JournaleLife
Volume6
DOIs
StatePublished - Jun 6 2017

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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