Transcriptional profiling and functional genomics reveal a role for AHR transcription factor in nephrogenesis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Transcriptional profiling and functional genomics experiments using E11.5 metanephros organ cultures from Ahr-/- and Ahr+/+ have shown that aryl hydrocarbon receptor (AHR) transcription factor is involved in the regulation of mesenchymal-to-epithelial transition (MET) during nephrogenesis. This response is mediated by alterations in the post-transcriptional control of Wilms' tumor suppressor (Wt1) gene and Wt1 splicing. In this article, biologically relevant gene predictor sets of the nephrogenic response were calculated for target genes of interest. The predictability of the gene set for each target was quantified by the coefficient of determination which provided a good criterion for identification of predictor sets that define the complex gene-gene interactions co-regulated by Ahr and Wt1. A subset of the signature genes was found to be co-regulated by Ahr and Wt1 and was responsible for shifts in renal cell transdifferentiation.

Original languageEnglish (US)
Title of host publicationAnnals of the New York Academy of Sciences
Pages728-735
Number of pages8
Volume1076
DOIs
StatePublished - Sep 2006
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1076
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

Aryl Hydrocarbon Receptors
Genomics
Transcription Factors
Genes
Cell Transdifferentiation
Wilms' Tumor Genes
Epithelial-Mesenchymal Transition
Organ Culture Techniques
Tumor Suppressor Genes
Tumors
Kidney

Keywords

  • Aryl hydrocarbon receptor
  • Development
  • Functional genomics
  • Nephrogenesis
  • Wilm's tumor suppressor gene

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ramos, K. (2006). Transcriptional profiling and functional genomics reveal a role for AHR transcription factor in nephrogenesis. In Annals of the New York Academy of Sciences (Vol. 1076, pp. 728-735). (Annals of the New York Academy of Sciences; Vol. 1076). https://doi.org/10.1196/annals.1371.045

Transcriptional profiling and functional genomics reveal a role for AHR transcription factor in nephrogenesis. / Ramos, Kenneth.

Annals of the New York Academy of Sciences. Vol. 1076 2006. p. 728-735 (Annals of the New York Academy of Sciences; Vol. 1076).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ramos, K 2006, Transcriptional profiling and functional genomics reveal a role for AHR transcription factor in nephrogenesis. in Annals of the New York Academy of Sciences. vol. 1076, Annals of the New York Academy of Sciences, vol. 1076, pp. 728-735. https://doi.org/10.1196/annals.1371.045
Ramos K. Transcriptional profiling and functional genomics reveal a role for AHR transcription factor in nephrogenesis. In Annals of the New York Academy of Sciences. Vol. 1076. 2006. p. 728-735. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1371.045
Ramos, Kenneth. / Transcriptional profiling and functional genomics reveal a role for AHR transcription factor in nephrogenesis. Annals of the New York Academy of Sciences. Vol. 1076 2006. pp. 728-735 (Annals of the New York Academy of Sciences).
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