Transcriptome-derived evidence supports recent polyploidization and a major phylogeographic division in Trithuria submersa (Hydatellaceae, Nymphaeales)

Isabel Marques, Sean A. Montgomery, Michael S Barker, Terry D. Macfarlane, John G. Conran, Pilar Catalán, Loren H. Rieseberg, Paula J. Rudall, Sean W. Graham

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Relatively little is known about species-level genetic diversity in flowering plants outside the eudicots and monocots, and it is often unclear how to interpret genetic patterns in lineages with whole-genome duplications. We addressed these issues in a polyploid representative of Hydatellaceae, part of the water-lily order Nymphaeales. We examined a transcriptome of Trithuria submersa for evidence of recent whole-genome duplication, and applied transcriptome-derived microsatellite (expressed-sequence tag simple-sequence repeat (EST-SSR)) primers to survey genetic variation in populations across its range in mainland Australia. A transcriptome-based Ks plot revealed at least one recent polyploidization event, consistent with fixed heterozygous genotypes representing underlying sets of homeologous loci. A strong genetic division coincides with a trans-Nullarbor biogeographic boundary. Patterns of 'allelic' variation (no more than two variants per EST-SSR genotype) and recently published chromosomal evidence are consistent with the predicted polyploidization event and substantial homozygosity underlying fixed heterozygote SSR genotypes, which in turn reflect a selfing mating system. The Nullarbor Plain is a barrier to gene flow between two deep lineages of T. submersa that may represent cryptic species. The markers developed here should also be useful for further disentangling species relationships, and provide a first step towards future genomic studies in Trithuria.

Original languageEnglish (US)
Pages (from-to)310-323
Number of pages14
JournalNew Phytologist
Volume210
Issue number1
DOIs
StatePublished - Apr 1 2016

Fingerprint

Hydatellaceae
Nymphaeales
Transcriptome
transcriptome
Microsatellite Repeats
Expressed Sequence Tags
Genotype
microsatellite repeats
expressed sequence tags
Nymphaeaceae
heterozygosity
Genome
water lilies
genetic variation
Polyploidy
genome
Gene Flow
genotype
Heterozygote
homozygosity

Keywords

  • Trithuria
  • ANA-grade
  • Australia
  • Microsatellite markers
  • Population genetic diversity
  • Trans-Nullarbor plants
  • Transcriptome-enabled markers

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Transcriptome-derived evidence supports recent polyploidization and a major phylogeographic division in Trithuria submersa (Hydatellaceae, Nymphaeales). / Marques, Isabel; Montgomery, Sean A.; Barker, Michael S; Macfarlane, Terry D.; Conran, John G.; Catalán, Pilar; Rieseberg, Loren H.; Rudall, Paula J.; Graham, Sean W.

In: New Phytologist, Vol. 210, No. 1, 01.04.2016, p. 310-323.

Research output: Contribution to journalArticle

Marques, I, Montgomery, SA, Barker, MS, Macfarlane, TD, Conran, JG, Catalán, P, Rieseberg, LH, Rudall, PJ & Graham, SW 2016, 'Transcriptome-derived evidence supports recent polyploidization and a major phylogeographic division in Trithuria submersa (Hydatellaceae, Nymphaeales)', New Phytologist, vol. 210, no. 1, pp. 310-323. https://doi.org/10.1111/nph.13755
Marques, Isabel ; Montgomery, Sean A. ; Barker, Michael S ; Macfarlane, Terry D. ; Conran, John G. ; Catalán, Pilar ; Rieseberg, Loren H. ; Rudall, Paula J. ; Graham, Sean W. / Transcriptome-derived evidence supports recent polyploidization and a major phylogeographic division in Trithuria submersa (Hydatellaceae, Nymphaeales). In: New Phytologist. 2016 ; Vol. 210, No. 1. pp. 310-323.
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