Transcriptome profile of a bovine respiratory disease pathogen

Mannheimia haemolytica PHL213.

Joseph S. Reddy, Ranjit Kumar, James M. Watt, Mark L. Lawrence, Shane C Burgess, Bindu Nanduri

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Computational methods for structural gene annotation have propelled gene discovery but face certain drawbacks with regards to prokaryotic genome annotation. Identification of transcriptional start sites, demarcating overlapping gene boundaries, and identifying regulatory elements such as small RNA are not accurate using these approaches. In this study, we re-visit the structural annotation of Mannheimia haemolytica PHL213, a bovine respiratory disease pathogen. M. haemolytica is one of the causative agents of bovine respiratory disease that results in about $3 billion annual losses to the cattle industry. We used RNA-Seq and analyzed the data using freely-available computational methods and resources. The aim was to identify previously unannotated regions of the genome using RNA-Seq based expression profile to complement the existing annotation of this pathogen. Using the Illumina Genome Analyzer, we generated 9,055,826 reads (average length ~76 bp) and aligned them to the reference genome using Bowtie. The transcribed regions were analyzed using SAMTOOLS and custom Perl scripts in conjunction with BLAST searches and available gene annotation information. The single nucleotide resolution map enabled the identification of 14 novel protein coding regions as well as 44 potential novel sRNA. The basal transcription profile revealed that 2,506 of the 2,837 annotated regions were expressed in vitro, at 95.25% coverage, representing all broad functional gene categories in the genome. The expression profile also helped identify 518 potential operon structures involving 1,086 co-expressed pairs. We also identified 11 proteins with mutated/alternate start codons. The application of RNA-Seq based transcriptome profiling to structural gene annotation helped correct existing annotation errors and identify potential novel protein coding regions and sRNA. We used computational tools to predict regulatory elements such as promoters and terminators associated with the novel expressed regions for further characterization of these novel functional elements. Our study complements the existing structural annotation of Mannheimia haemolytica PHL213 based on experimental evidence. Given the role of sRNA in virulence gene regulation and stress response, potential novel sRNA described in this study can form the framework for future studies to determine the role of sRNA, if any, in M. haemolytica pathogenesis.

Original languageEnglish (US)
JournalBMC Bioinformatics
Volume13 Suppl 15
StatePublished - 2012
Externally publishedYes

Fingerprint

Mannheimia haemolytica
Cattle Diseases
Pulmonary diseases
Pathogens
Transcriptome
Annotation
Molecular Sequence Annotation
Genes
Genome
RNA
Gene
Open Reading Frames
Overlapping Genes
Protein
Computational Methods
Initiator Codon
Genetic Association Studies
Gene Expression Profiling
Operon
Complement

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Applied Mathematics
  • Structural Biology

Cite this

Reddy, J. S., Kumar, R., Watt, J. M., Lawrence, M. L., Burgess, S. C., & Nanduri, B. (2012). Transcriptome profile of a bovine respiratory disease pathogen: Mannheimia haemolytica PHL213. BMC Bioinformatics, 13 Suppl 15.

Transcriptome profile of a bovine respiratory disease pathogen : Mannheimia haemolytica PHL213. / Reddy, Joseph S.; Kumar, Ranjit; Watt, James M.; Lawrence, Mark L.; Burgess, Shane C; Nanduri, Bindu.

In: BMC Bioinformatics, Vol. 13 Suppl 15, 2012.

Research output: Contribution to journalArticle

Reddy, JS, Kumar, R, Watt, JM, Lawrence, ML, Burgess, SC & Nanduri, B 2012, 'Transcriptome profile of a bovine respiratory disease pathogen: Mannheimia haemolytica PHL213.', BMC Bioinformatics, vol. 13 Suppl 15.
Reddy, Joseph S. ; Kumar, Ranjit ; Watt, James M. ; Lawrence, Mark L. ; Burgess, Shane C ; Nanduri, Bindu. / Transcriptome profile of a bovine respiratory disease pathogen : Mannheimia haemolytica PHL213. In: BMC Bioinformatics. 2012 ; Vol. 13 Suppl 15.
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