Transcriptome profiling of a toxic dinoflagellate reveals a gene-rich protist and a potential impact on gene expression due to bacterial presence

Ahmed Moustafa, Andrew N. Evans, David M. Kulis, Jeremiah Hackett, Deana L. Erdner, Donald M. Anderson, Debashish Bhattacharya

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Background: Dinoflagellates are unicellular, often photosynthetic protists that play a major role in the dynamics of the Earth's oceans and climate. Sequencing of dinoflagellate nuclear DNA is thwarted by their massive genome sizes that are often several times that in humans. However, modern transcriptomic methods offer promising approaches to tackle this challenging system. Here, we used massively parallel signature sequencing (MPSS) to understand global transcriptional regulation patterns in Alexandrium tamarense cultures that were grown under four different conditions. Methodology/Principal Findings: We generated more than 40,000 unique short expression signatures gathered from the four conditions. Of these, about 11,000 signatures did not display detectable differential expression patterns. At a p-value <, 1E-10, 1,124 signatures were differentially expressed in the three treatments, xenic, nitrogen-limited, and phosphoruslimited, compared to the nutrient-replete control, with the presence of bacteria explaining the largest set of these differentially expressed signatures. Conclusions/Significance: Among microbial eukaryotes, dinoflagellates contain the largest number of genes in their nuclear genomes. These genes occur in complex families, many of which have evolved via recent gene duplication events. Our expression data suggest that about 73% of the Alexandrium transcriptome shows no significant change in gene expression under the experimental conditions used here and may comprise a "core" component for this species. We report a fundamental shift in expression patterns in response to the presence of bacteria, highlighting the impact of biotic interaction on gene expression in dinoflagellates.

Original languageEnglish (US)
Article numbere9688
JournalPLoS One
Volume5
Issue number3
DOIs
StatePublished - Mar 12 2010

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Dinoflagellida
Poisons
Gene Expression Profiling
transcriptomics
Gene expression
Genes
Gene Expression
gene expression
Alexandrium
nuclear genome
genes
Bacteria
Genome Size
High-Throughput Nucleotide Sequencing
Gene Duplication
bacteria
gene duplication
Eukaryota
Climate
Transcriptome

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Transcriptome profiling of a toxic dinoflagellate reveals a gene-rich protist and a potential impact on gene expression due to bacterial presence. / Moustafa, Ahmed; Evans, Andrew N.; Kulis, David M.; Hackett, Jeremiah; Erdner, Deana L.; Anderson, Donald M.; Bhattacharya, Debashish.

In: PLoS One, Vol. 5, No. 3, e9688, 12.03.2010.

Research output: Contribution to journalArticle

Moustafa, Ahmed ; Evans, Andrew N. ; Kulis, David M. ; Hackett, Jeremiah ; Erdner, Deana L. ; Anderson, Donald M. ; Bhattacharya, Debashish. / Transcriptome profiling of a toxic dinoflagellate reveals a gene-rich protist and a potential impact on gene expression due to bacterial presence. In: PLoS One. 2010 ; Vol. 5, No. 3.
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