Transcriptome response to heat stress in a chicken hepatocellular carcinoma cell line

Liang Sun, Susan J. Lamont, Amanda M. Cooksey, Fiona M McCarthy, Catalina O. Tudor, K. Vijay-Shanker, Rachael M. DeRita, Max Rothschild, Chris Ashwell, Michael E. Persia, Carl J. Schmidt

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Heat stress triggers an evolutionarily conserved set of responses in cells. The transcriptome responds to hyperthermia by altering expression of genes to adapt the cell or organism to survive the heat challenge. RNA-seq technology allows rapid identification of environmentally responsive genes on a large scale. In this study, we have used RNA-seq to identify heat stress responsive genes in the chicken male white leghorn hepatocellular (LMH) cell line. The transcripts of 812 genes were responsive to heat stress (p <0.01) with 235 genes upregulated and 577 downregulated following 2.5 h of heat stress. Among the upregulated were genes whose products function as chaperones, along with genes affecting collagen synthesis and deposition, transcription factors, chromatin remodelers, and genes modulating the WNT and TGF-beta pathways. Predominant among the downregulated genes were ones that affect DNA replication and repair along with chromosomal segregation. Many of the genes identified in this study have not been previously implicated in the heat stress response. These data extend our understanding of the transcriptome response to heat stress with many of the identified biological processes and pathways likely to function in adapting cells and organisms to hyperthermic stress. Furthermore, this study should provide important insight to future efforts attempting to improve species abilities to withstand heat stress through genome-wide association studies and breeding.

Original languageEnglish (US)
Pages (from-to)939-950
Number of pages12
JournalCell Stress and Chaperones
Volume20
Issue number6
DOIs
StatePublished - Nov 1 2015

Fingerprint

Heat-Shock Response
Transcriptome
Hepatocellular Carcinoma
Chickens
Genes
Cells
Cell Line
Hot Temperature
Down-Regulation
RNA
Biological Phenomena
Genome-Wide Association Study
DNA Replication
DNA Repair
Transforming Growth Factor beta
Chromatin
Breeding
Fever
Transcription Factors
Collagen

Keywords

  • Chicken
  • Heat stress
  • LMH cells
  • RNA-seq
  • Transcriptome

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Sun, L., Lamont, S. J., Cooksey, A. M., McCarthy, F. M., Tudor, C. O., Vijay-Shanker, K., ... Schmidt, C. J. (2015). Transcriptome response to heat stress in a chicken hepatocellular carcinoma cell line. Cell Stress and Chaperones, 20(6), 939-950. https://doi.org/10.1007/s12192-015-0621-0

Transcriptome response to heat stress in a chicken hepatocellular carcinoma cell line. / Sun, Liang; Lamont, Susan J.; Cooksey, Amanda M.; McCarthy, Fiona M; Tudor, Catalina O.; Vijay-Shanker, K.; DeRita, Rachael M.; Rothschild, Max; Ashwell, Chris; Persia, Michael E.; Schmidt, Carl J.

In: Cell Stress and Chaperones, Vol. 20, No. 6, 01.11.2015, p. 939-950.

Research output: Contribution to journalArticle

Sun, L, Lamont, SJ, Cooksey, AM, McCarthy, FM, Tudor, CO, Vijay-Shanker, K, DeRita, RM, Rothschild, M, Ashwell, C, Persia, ME & Schmidt, CJ 2015, 'Transcriptome response to heat stress in a chicken hepatocellular carcinoma cell line', Cell Stress and Chaperones, vol. 20, no. 6, pp. 939-950. https://doi.org/10.1007/s12192-015-0621-0
Sun, Liang ; Lamont, Susan J. ; Cooksey, Amanda M. ; McCarthy, Fiona M ; Tudor, Catalina O. ; Vijay-Shanker, K. ; DeRita, Rachael M. ; Rothschild, Max ; Ashwell, Chris ; Persia, Michael E. ; Schmidt, Carl J. / Transcriptome response to heat stress in a chicken hepatocellular carcinoma cell line. In: Cell Stress and Chaperones. 2015 ; Vol. 20, No. 6. pp. 939-950.
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