Transgenesis by means of blastocyst-derived embryonic stem cell lines

A. Gossler, Thomas C Doetschman, R. Korn, E. Serfling, R. Kemler

Research output: Contribution to journalArticle

317 Citations (Scopus)

Abstract

This study demonstrates that blastocyst-derived embryonic stem cells (ES cells) can be used as a vehicle for transgenesis. The method is nearly as efficient as other methods, and the introduced neomycin phosphotransferase (neo) gene is stably transmitted through several generations with no apparent loss in G418 resistance. An important factor contributing to the efficiency of this process is the rigorous selection, before blastocyst injection, of genetically transformed cells for in vitro developmental pluripotency. One of the advantages of the ES cell route to transgenesis is that it provides investigators with the opportunity to screen for the desired genetic alterations before reintroducing the ES cells into the animal.

Original languageEnglish (US)
Pages (from-to)9065-9069
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number23
StatePublished - 1986
Externally publishedYes

Fingerprint

Gene Transfer Techniques
Blastocyst
Embryonic Stem Cells
Cell Line
Kanamycin Kinase
Research Personnel
Injections
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Transgenesis by means of blastocyst-derived embryonic stem cell lines. / Gossler, A.; Doetschman, Thomas C; Korn, R.; Serfling, E.; Kemler, R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 23, 1986, p. 9065-9069.

Research output: Contribution to journalArticle

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