Transgenic approaches for modifying the mammary gland to produce therapeutic proteins

D. Romagnolo, R. P. DiAugustine

Research output: Contribution to journalReview article

2 Scopus citations

Abstract

Bioengineering of the mammary gland to produce proteins of therapeutic and industrial value is the result of extensive investigation of thy physiology of the mammary gland and the ability to generate transgenic animals. Targeting the expression of heterologous proteins to mammary tissue requires a thorough understanding of the biochemical events that coordinate growth and differentiation of the mammary gland and of the hormonal and developmental regulation of expression of milk protein genes. The characterization of mammary-specific promoter regions in milk protein genes and knowledge of the mechanisms that confer integration site-independent expression of transgenes have significantly contributed to modifying the mammary gland to produce heterologous proteins of therapeutic interest. The generation of large transgenic farm animals provides the opportunity for large-scale production of proteins in milk that have a therapeutic value are naturally present at low concentrations in biological fluids. Transgenic mammary epithelial cells offer a versatile research model in biomedical, environmental health, and neonatal toxicology research.

Original languageEnglish (US)
Pages (from-to)846-851
Number of pages6
JournalEnvironmental health perspectives
Volume102
Issue number10
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Keywords

  • Chronic and hereditary diseases
  • Environmental and health research
  • Gene expression
  • Lactation
  • Mammary gland
  • Therapeutic proteins
  • Transgenics

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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