Transgenic Evaluation of Activated Mutant Alleles of SOS2 Reveals a Critical Requirement for Its Kinase Activity and C-Terminal Regulatory Domain for Salt Tolerance in Arabidopsis thaliana

Yan Guo, Quan Sheng Qiu, Francisco J. Quintero, José M. Pardo, Masaru Ohta, Changqing Zhang, Karen S Schumaker, Jian Kang Zhu

Research output: Contribution to journalArticle

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Abstract

In Arabidopsis thaliana, the calcium binding protein Salt Overly Sensitives (SOS3) interacts with and activates the protein kinase SOS2, which in turn activates the plasma membrane Na+/H+ antiporter SOS1 to bring about sodium ion homeostasis and salt tolerance. Constitutively active alleles of SOS2 can be constructed in vitro by changing Thr 168 to Asp in the activation loop of the kinase catalytic domain and/or by removing the autoinhibitory FISL motif from the C-terminal regulatory domain. We expressed various activated forms of SOS2 in Saccharomyces cerevisiae (yeast) and in A. thaliana and evaluated the salt tolerance of the transgenic organisms. Experiments in which the activated SOS2 alleles were coexpressed with SOS2 in S. cerevis/ae showed that the kinase activity of SOS2 is partially sufficient for SOS1 activation in vivo, and higher kinase activity leads to greater SOS1 activation. Coexpression of SOS3 with SOS2 forms that retained the FISL motif resulted in more dramatic increases in salt tolerance. In planta assays showed that the Thr168-to-Asp-activated mutant SOS2 partially rescued the salt hypersensitivity in sos2 and sos3 mutant plants. By contrast, SOS2 lacking only the FISL domain suppressed the sos2 but not the sos3 mutation, whereas truncated forms in which the C terminus had been removed could not restore the growth of either sos2 or sos3 plants. Expression of some of the activated SOS2 proteins in wild-type A. thaliana conferred increased satt tolerance. These studies demonstrate that the protein kinase activity of SOS2 is partially sufficient for activation of SOS1 and for salt tolerance in vivo and in planta and that the kinase activity of SOS2 is limiting for plant salt tolerance. The results also reveal an essential in planta role for the SOS2 C-terminal regulatory domain in salt tolerance.

Original languageEnglish (US)
Pages (from-to)435-449
Number of pages15
JournalPlant Cell
Volume16
Issue number2
DOIs
StatePublished - Feb 2004

Fingerprint

Salt-Tolerance
salt tolerance
Arabidopsis
phosphotransferases (kinases)
Phosphotransferases
Arabidopsis thaliana
Salts
Alleles
genetically modified organisms
alleles
mutants
Plantae
Chemical activation
protein kinases
Protein Kinases
Genetically Modified Organisms
Yeast
salts
calcium-binding proteins
Sodium-Hydrogen Antiporter

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Transgenic Evaluation of Activated Mutant Alleles of SOS2 Reveals a Critical Requirement for Its Kinase Activity and C-Terminal Regulatory Domain for Salt Tolerance in Arabidopsis thaliana. / Guo, Yan; Qiu, Quan Sheng; Quintero, Francisco J.; Pardo, José M.; Ohta, Masaru; Zhang, Changqing; Schumaker, Karen S; Zhu, Jian Kang.

In: Plant Cell, Vol. 16, No. 2, 02.2004, p. 435-449.

Research output: Contribution to journalArticle

Guo, Yan ; Qiu, Quan Sheng ; Quintero, Francisco J. ; Pardo, José M. ; Ohta, Masaru ; Zhang, Changqing ; Schumaker, Karen S ; Zhu, Jian Kang. / Transgenic Evaluation of Activated Mutant Alleles of SOS2 Reveals a Critical Requirement for Its Kinase Activity and C-Terminal Regulatory Domain for Salt Tolerance in Arabidopsis thaliana. In: Plant Cell. 2004 ; Vol. 16, No. 2. pp. 435-449.
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abstract = "In Arabidopsis thaliana, the calcium binding protein Salt Overly Sensitives (SOS3) interacts with and activates the protein kinase SOS2, which in turn activates the plasma membrane Na+/H+ antiporter SOS1 to bring about sodium ion homeostasis and salt tolerance. Constitutively active alleles of SOS2 can be constructed in vitro by changing Thr 168 to Asp in the activation loop of the kinase catalytic domain and/or by removing the autoinhibitory FISL motif from the C-terminal regulatory domain. We expressed various activated forms of SOS2 in Saccharomyces cerevisiae (yeast) and in A. thaliana and evaluated the salt tolerance of the transgenic organisms. Experiments in which the activated SOS2 alleles were coexpressed with SOS2 in S. cerevis/ae showed that the kinase activity of SOS2 is partially sufficient for SOS1 activation in vivo, and higher kinase activity leads to greater SOS1 activation. Coexpression of SOS3 with SOS2 forms that retained the FISL motif resulted in more dramatic increases in salt tolerance. In planta assays showed that the Thr168-to-Asp-activated mutant SOS2 partially rescued the salt hypersensitivity in sos2 and sos3 mutant plants. By contrast, SOS2 lacking only the FISL domain suppressed the sos2 but not the sos3 mutation, whereas truncated forms in which the C terminus had been removed could not restore the growth of either sos2 or sos3 plants. Expression of some of the activated SOS2 proteins in wild-type A. thaliana conferred increased satt tolerance. These studies demonstrate that the protein kinase activity of SOS2 is partially sufficient for activation of SOS1 and for salt tolerance in vivo and in planta and that the kinase activity of SOS2 is limiting for plant salt tolerance. The results also reveal an essential in planta role for the SOS2 C-terminal regulatory domain in salt tolerance.",
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AU - Guo, Yan

AU - Qiu, Quan Sheng

AU - Quintero, Francisco J.

AU - Pardo, José M.

AU - Ohta, Masaru

AU - Zhang, Changqing

AU - Schumaker, Karen S

AU - Zhu, Jian Kang

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AB - In Arabidopsis thaliana, the calcium binding protein Salt Overly Sensitives (SOS3) interacts with and activates the protein kinase SOS2, which in turn activates the plasma membrane Na+/H+ antiporter SOS1 to bring about sodium ion homeostasis and salt tolerance. Constitutively active alleles of SOS2 can be constructed in vitro by changing Thr 168 to Asp in the activation loop of the kinase catalytic domain and/or by removing the autoinhibitory FISL motif from the C-terminal regulatory domain. We expressed various activated forms of SOS2 in Saccharomyces cerevisiae (yeast) and in A. thaliana and evaluated the salt tolerance of the transgenic organisms. Experiments in which the activated SOS2 alleles were coexpressed with SOS2 in S. cerevis/ae showed that the kinase activity of SOS2 is partially sufficient for SOS1 activation in vivo, and higher kinase activity leads to greater SOS1 activation. Coexpression of SOS3 with SOS2 forms that retained the FISL motif resulted in more dramatic increases in salt tolerance. In planta assays showed that the Thr168-to-Asp-activated mutant SOS2 partially rescued the salt hypersensitivity in sos2 and sos3 mutant plants. By contrast, SOS2 lacking only the FISL domain suppressed the sos2 but not the sos3 mutation, whereas truncated forms in which the C terminus had been removed could not restore the growth of either sos2 or sos3 plants. Expression of some of the activated SOS2 proteins in wild-type A. thaliana conferred increased satt tolerance. These studies demonstrate that the protein kinase activity of SOS2 is partially sufficient for activation of SOS1 and for salt tolerance in vivo and in planta and that the kinase activity of SOS2 is limiting for plant salt tolerance. The results also reveal an essential in planta role for the SOS2 C-terminal regulatory domain in salt tolerance.

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