Transient cardiac expression of the tinman-family homeobox gene, XNkx2- 10

Craig S. Newman, James Reecy, Matt W. Grow, Karen Ni, Thomas Boettger, Michael Kessel, Robert J. Schwartz, Paul A Krieg

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In Drosophila, the tinman homeobox gene is absolutely required for heart development. In the vertebrates, a small family of tinman-related genes, the cardiac NK-2 genes, appear to play a similar role in the formation of the vertebrate heart. However, targeted gene ablation of one of these genes, Nkx2-5, results in defects in only the late stages of cardiac development suggesting the presence of a rescuing gene function early in development. Here, we report the characterization of a novel tinman-related gene, XNkx2- 10, which is expressed during early heart development in Xenopus. Using in vitro assays, we show that XNkx2-10 is capable of transactivating expression from promoters previously shown to be activated by other tinman-related genes, including Nkx2-5. Furthermore, Xenopus Nkx2-10 can synergize with the GATA-4 and SRF transcription factors to activate reporter gene expression. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)369-373
Number of pages5
JournalMechanisms of Development
Volume91
Issue number1-2
DOIs
StatePublished - Mar 1 2000
Externally publishedYes

Fingerprint

Homeobox Genes
Genes
Xenopus
Vertebrates
GATA4 Transcription Factor
Reporter Genes
Drosophila
Gene Expression

Keywords

  • Heart
  • Nkx
  • Pharyngeal endoderm
  • tinman

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Transient cardiac expression of the tinman-family homeobox gene, XNkx2- 10. / Newman, Craig S.; Reecy, James; Grow, Matt W.; Ni, Karen; Boettger, Thomas; Kessel, Michael; Schwartz, Robert J.; Krieg, Paul A.

In: Mechanisms of Development, Vol. 91, No. 1-2, 01.03.2000, p. 369-373.

Research output: Contribution to journalArticle

Newman, CS, Reecy, J, Grow, MW, Ni, K, Boettger, T, Kessel, M, Schwartz, RJ & Krieg, PA 2000, 'Transient cardiac expression of the tinman-family homeobox gene, XNkx2- 10', Mechanisms of Development, vol. 91, no. 1-2, pp. 369-373. https://doi.org/10.1016/S0925-4773(99)00291-9
Newman, Craig S. ; Reecy, James ; Grow, Matt W. ; Ni, Karen ; Boettger, Thomas ; Kessel, Michael ; Schwartz, Robert J. ; Krieg, Paul A. / Transient cardiac expression of the tinman-family homeobox gene, XNkx2- 10. In: Mechanisms of Development. 2000 ; Vol. 91, No. 1-2. pp. 369-373.
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