Translation against an applied force

Gary M. Skinner, Yeonee Seol, Koen Visscher

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Ribosome structure and mechanism are largely conserved among all known forms of life. Therefore, the motions associated with translation may be among the most ancient and fundamental in biology. However, the molecular mechanism of translocation, the coordinated movement of tRNAs and associated mRNA on the ribosome, has eluded scientists and remains obscure. Single-molecule experiments using optical tweezers and fluorescence microscope are starting to shed new light on these questions. For example, we have observed that moderate forces reverse direction of motion and ribosomes seem to slip backward into the 5' direction along a poly(U) message. Although the detailed molecular mechanism for ribosome slippage is not fully understood, these observations raise interesting biological questions about e.g. -1 frameshifting. Is the -1 frameshift essential for HIV-1 replication a result of tension in the message? Single-molecule experiments open the way towards quantitative modeling of ribosome motion and related phenomena such as -1 frameshifting.

Original languageEnglish (US)
Title of host publicationAIP Conference Proceedings
Pages1-10
Number of pages10
Volume978
DOIs
StatePublished - 2007
Event3rd Mexican Meeting on Mathematical and Experimental Physics - Mexico City, Mexico
Duration: Sep 10 2007Sep 14 2007

Other

Other3rd Mexican Meeting on Mathematical and Experimental Physics
CountryMexico
CityMexico City
Period9/10/079/14/07

Fingerprint

ribosomes
messages
human immunodeficiency virus
biology
molecules
slip
microscopes
fluorescence

Keywords

  • RNA elasticity
  • Single-molecule
  • Translation

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Skinner, G. M., Seol, Y., & Visscher, K. (2007). Translation against an applied force. In AIP Conference Proceedings (Vol. 978, pp. 1-10) https://doi.org/10.1063/1.2891411

Translation against an applied force. / Skinner, Gary M.; Seol, Yeonee; Visscher, Koen.

AIP Conference Proceedings. Vol. 978 2007. p. 1-10.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Skinner, GM, Seol, Y & Visscher, K 2007, Translation against an applied force. in AIP Conference Proceedings. vol. 978, pp. 1-10, 3rd Mexican Meeting on Mathematical and Experimental Physics, Mexico City, Mexico, 9/10/07. https://doi.org/10.1063/1.2891411
Skinner GM, Seol Y, Visscher K. Translation against an applied force. In AIP Conference Proceedings. Vol. 978. 2007. p. 1-10 https://doi.org/10.1063/1.2891411
Skinner, Gary M. ; Seol, Yeonee ; Visscher, Koen. / Translation against an applied force. AIP Conference Proceedings. Vol. 978 2007. pp. 1-10
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