Transmitter release face Ca2+ channel clusters persist at isolated presynaptic terminals

Li Sun, Qi Li, Rajesh Khanna, Allen W. Chan, Fiona Wong, Elise F. Stanley

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Ca2+ influx through N-type Ca2+ channels (CaV2.2) is known to be critical for transmitter release at many synapses. These channels are known to be localized to transmitter release sites, but their anchoring mechanism remains unknown. Recent studies have demonstrated that presynaptic organization is subject to interactions with the postsynaptic cell or the intervening extracellular matrix. We used a previously described high-affinity antibody against the N-type Ca2+ channels, Ab571, to localize Ca 2+ channel clusters at the release face of an isolated giant calyx-type synapse to test whether the maintenance of these clusters requires an intact extracellular matrix or contact with the postsynaptic cell. Because the number of Ca2+ channel clusters was unchanged after extracellular matrix dispersal or nerve terminal isolation, we conclude that presynaptic transmitter release face Ca2+ clusters can be maintained independently of extracellular influences. Our results suggest that a presynaptic molecular scaffold is responsible for the maintenance of release site Ca2+ channel clusters.

Original languageEnglish (US)
Pages (from-to)1391-1396
Number of pages6
JournalEuropean Journal of Neuroscience
Volume23
Issue number5
DOIs
StatePublished - Mar 1 2006
Externally publishedYes

Keywords

  • Active zone
  • CaV2.2
  • Calyx
  • Chick ciliary ganglion
  • Extracellular matrix
  • Scaffold

ASJC Scopus subject areas

  • Neuroscience(all)

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