Transport of glutamine in rat intestinal brush-border membrane vesicles

Kent Van Voorhis, Hamid M. Said, Fayez K Ghishan, Naji N. Abumrad

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Transport of glutamine across the brush-border membrane of the rat intestine was examined using brush-border membrane vesicle (BBMV) technique. Osmolarity and temperature studies indicated that the uptake of glutamine by BBMV is mostly the result of transport of the substrate into the intravesicular space. Transport of glutamine was Na+-gradient dependent (out > in) with a distinct 'overshoot' phenomenon. Initial rate of transport of glutamine as a function of concentration was saturable both in the presence and absence of a Na+ gradient (out > in). Apparent Km of 3.50 and 3.34 mM and Vmax of 707 and 282 pmol/mg protein per 7 s, were calculated for the Na+-dependent and the Na+-independent transport processes of glutamine. The transport of [3H]glutamine by the Na+-dependent and the Na+-independent processes was severely inhibited by the addition to the incubation medium of other amino acids and unlabelled glutamine. Inducing a relatively negative intravesicular compartment with the use of valinomycin and an outwardly directed K+ gradient stimulated glutamine transport. This indicates that transport of the substrate by the Na+-dependent process is electrogenic in nature. Transport of glutamine by the Na+-independent process, however, appeared to be electroneutral in nature. These results demonstrate the existence of two carrier-mediated transport processes for glutamine in the rat intestinal BBMV, one is Na+-dependent and the other is Na+-independent. Furthermore, the results suggest that glutamine transport by the Na+-dependent process probably occurs by a glutamine/Na+ cotransport mechanism.

Original languageEnglish (US)
Pages (from-to)51-55
Number of pages5
JournalBBA - Biomembranes
Volume978
Issue number1
DOIs
StatePublished - Jan 16 1989
Externally publishedYes

Fingerprint

Brushes
Microvilli
Glutamine
Rats
Membranes
Valinomycin
Carrier transport
Substrates
Osmolar Concentration
Intestines
Amino Acids

Keywords

  • (Rat jejunum)
  • Brush-border membrane
  • Glutamine transport

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Transport of glutamine in rat intestinal brush-border membrane vesicles. / Van Voorhis, Kent; Said, Hamid M.; Ghishan, Fayez K; Abumrad, Naji N.

In: BBA - Biomembranes, Vol. 978, No. 1, 16.01.1989, p. 51-55.

Research output: Contribution to journalArticle

Van Voorhis, Kent ; Said, Hamid M. ; Ghishan, Fayez K ; Abumrad, Naji N. / Transport of glutamine in rat intestinal brush-border membrane vesicles. In: BBA - Biomembranes. 1989 ; Vol. 978, No. 1. pp. 51-55.
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