Transport of horseradish peroxidase across monkey trophoblastic epithelium in coated and uncoated vesicles

Jean Wilson, B. F. King

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This study used membranous chorion of the macaque monkey placenta to examine uptake and processing of exogenous proteins. Tissue was incubated with either cationic or anionic horseradish peroxidase. Incubation time was varied between 5-25 min to follow the endocytic pathways. In spite of some differences in binding, uptake and processing of the isozymes was similar. In the presence of tracers at 37°C both horseradish peroxidases were taken up in large (150-175) nm diameter) coated vesicles. In addition, coated tubules 300-400 nm in length and 50-100 nm in diameter were seen in the apical cytoplasm. Studies using ruthenium red indicated that the coated tubules were derived from long coated invaginations of the free surface that pinch off into the apical cytoplasm. Often, the tubules bud off small (85-105 nm diameter) protein-filled coated vesicles which traversed the cytoplasm and fused with the basal-lateral plasma membrane. In other cases, the tubules or vesicles lost their clathrin coats and fused to form larger endocytic vesicles which later fused with phagolysosomes. After longer incubation, larger uncoated vesicles (endosomes) were seen releasing their contents at the basal-lateral membrane. These results suggest that multiple transport pathways exist in this epithelium. The first, involving only coated structures, may function to sort and concentrate specific ligands important for embryonic development. The second, involving the formation and translocation of large uncoated vesicles to the basal-lateral membrane, may also provide nutrients to the embryo. A third pathway directs the protein to phagolysosomes where it is presumably degraded. Degradation products could be used by the cells of the membranous chorion or may provide nutrients to the embryo.

Original languageEnglish (US)
Pages (from-to)174-183
Number of pages10
JournalAnatomical Record
Volume211
Issue number2
StatePublished - 1985
Externally publishedYes

Fingerprint

Coated Vesicles
Horseradish Peroxidase
vesicle
Haplorhini
monkeys
coated vesicles
Chorion
phagosomes
Phagosomes
peroxidase
Cytoplasm
epithelium
chorion
Epithelium
cytoplasm
embryo (animal)
Embryonic Structures
uptake mechanisms
Ruthenium Red
clathrin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Transport of horseradish peroxidase across monkey trophoblastic epithelium in coated and uncoated vesicles. / Wilson, Jean; King, B. F.

In: Anatomical Record, Vol. 211, No. 2, 1985, p. 174-183.

Research output: Contribution to journalArticle

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