Treatment of anthranilic acid in an anaerobic expanded granular sludge bed reactor at low concentrations

Elías Razo-Flores, Patrick Smulders, Francesc Prenafeta-Boldú, Gatze Lettinga, James A Field

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The mineralization of anthranilic acid (2AB) as the only carbon and energy source was studied in batch and continuous conditions using methanogenic granular sludge. Under batch conditions in serum vials, 2AB (300 mg/l) was completely mineralized to methane within 55 days time. The experiment with the anaerobic continuous expanded granular sludge bed (EGSB) reactor was initially conducted at an upflow velocity (V(up)) of 5 m/h, a hydraulic retention time (HRT) of 12 h and an organic loading rate (OLR) of 1.5 g chemical oxygen demand (COD)/l-d. After 102 days, 2AB was not degraded at all and the reactor operation was shifted to batch mode by recycling the effluent. After some days, 2AB was completely mineralized and accumulation of flocculent sludge was observed. Batch biodegradability assays demonstrated that this flocculent biomass had a higher specific biodegradation rate compared to the granular sludge. During EGSB reactor operation, the flocculent biomass which was not attached to the granules probably washed-out under the applied hydrodynamic conditions. When the EGSB reactor was operated at V(up) of 2 m/h to favour the retention of the flocculent biomass, 2AB was mineralized even at influent concentrations as low as 140 mg COD/L. Kinetic calculations indicated that the sludge had an apparent K(s) value for the mineralization of 2AB as low as 24 mg COD/l.

Original languageEnglish (US)
Pages (from-to)187-194
Number of pages8
JournalWater Science and Technology
Volume40
Issue number8
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Chemical oxygen demand
Reactor operation
Biomass
sludge
Acids
acid
chemical oxygen demand
Biodegradability
Biodegradation
Recycling
Effluents
Assays
Methane
Hydrodynamics
Hydraulics
biomass
Kinetics
Carbon
mineralization
reactor

Keywords

  • Anaerobic degradation
  • Anthranilic acid
  • Aromatic amines
  • EGSB reactor
  • Flocculent biomass
  • Granular sludge
  • Methanogenic conditions

ASJC Scopus subject areas

  • Water Science and Technology

Cite this

Treatment of anthranilic acid in an anaerobic expanded granular sludge bed reactor at low concentrations. / Razo-Flores, Elías; Smulders, Patrick; Prenafeta-Boldú, Francesc; Lettinga, Gatze; Field, James A.

In: Water Science and Technology, Vol. 40, No. 8, 1999, p. 187-194.

Research output: Contribution to journalArticle

Razo-Flores, Elías ; Smulders, Patrick ; Prenafeta-Boldú, Francesc ; Lettinga, Gatze ; Field, James A. / Treatment of anthranilic acid in an anaerobic expanded granular sludge bed reactor at low concentrations. In: Water Science and Technology. 1999 ; Vol. 40, No. 8. pp. 187-194.
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