Abstract
The mineralization of anthranilic acid (2AB) as the only carbon and energy source was studied in batch and continuous conditions using methanogenic granular sludge. Under batch conditions in serum vials, 2AB (300 mg/l) was completely mineralized to methane within 55 days time. The experiment with the anaerobic continuous expanded granular sludge bed (EGSB) reactor was initially conducted at an upflow velocity (V(up)) of 5 m/h, a hydraulic retention time (HRT) of 12 h and an organic loading rate (OLR) of 1.5 g chemical oxygen demand (COD)/l-d. After 102 days, 2AB was not degraded at all and the reactor operation was shifted to batch mode by recycling the effluent. After some days, 2AB was completely mineralized and accumulation of flocculent sludge was observed. Batch biodegradability assays demonstrated that this flocculent biomass had a higher specific biodegradation rate compared to the granular sludge. During EGSB reactor operation, the flocculent biomass which was not attached to the granules probably washed-out under the applied hydrodynamic conditions. When the EGSB reactor was operated at V(up) of 2 m/h to favour the retention of the flocculent biomass, 2AB was mineralized even at influent concentrations as low as 140 mg COD/L. Kinetic calculations indicated that the sludge had an apparent K(s) value for the mineralization of 2AB as low as 24 mg COD/l.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 187-194 |
| Number of pages | 8 |
| Journal | Water Science and Technology |
| Volume | 40 |
| Issue number | 8 |
| DOIs | |
| State | Published - 1999 |
| Externally published | Yes |
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Keywords
- Anaerobic degradation
- Anthranilic acid
- Aromatic amines
- EGSB reactor
- Flocculent biomass
- Granular sludge
- Methanogenic conditions
ASJC Scopus subject areas
- Water Science and Technology
Cite this
Treatment of anthranilic acid in an anaerobic expanded granular sludge bed reactor at low concentrations. / Razo-Flores, Elías; Smulders, Patrick; Prenafeta-Boldú, Francesc; Lettinga, Gatze; Field, James A.
In: Water Science and Technology, Vol. 40, No. 8, 1999, p. 187-194.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Treatment of anthranilic acid in an anaerobic expanded granular sludge bed reactor at low concentrations
AU - Razo-Flores, Elías
AU - Smulders, Patrick
AU - Prenafeta-Boldú, Francesc
AU - Lettinga, Gatze
AU - Field, James A
PY - 1999
Y1 - 1999
N2 - The mineralization of anthranilic acid (2AB) as the only carbon and energy source was studied in batch and continuous conditions using methanogenic granular sludge. Under batch conditions in serum vials, 2AB (300 mg/l) was completely mineralized to methane within 55 days time. The experiment with the anaerobic continuous expanded granular sludge bed (EGSB) reactor was initially conducted at an upflow velocity (V(up)) of 5 m/h, a hydraulic retention time (HRT) of 12 h and an organic loading rate (OLR) of 1.5 g chemical oxygen demand (COD)/l-d. After 102 days, 2AB was not degraded at all and the reactor operation was shifted to batch mode by recycling the effluent. After some days, 2AB was completely mineralized and accumulation of flocculent sludge was observed. Batch biodegradability assays demonstrated that this flocculent biomass had a higher specific biodegradation rate compared to the granular sludge. During EGSB reactor operation, the flocculent biomass which was not attached to the granules probably washed-out under the applied hydrodynamic conditions. When the EGSB reactor was operated at V(up) of 2 m/h to favour the retention of the flocculent biomass, 2AB was mineralized even at influent concentrations as low as 140 mg COD/L. Kinetic calculations indicated that the sludge had an apparent K(s) value for the mineralization of 2AB as low as 24 mg COD/l.
AB - The mineralization of anthranilic acid (2AB) as the only carbon and energy source was studied in batch and continuous conditions using methanogenic granular sludge. Under batch conditions in serum vials, 2AB (300 mg/l) was completely mineralized to methane within 55 days time. The experiment with the anaerobic continuous expanded granular sludge bed (EGSB) reactor was initially conducted at an upflow velocity (V(up)) of 5 m/h, a hydraulic retention time (HRT) of 12 h and an organic loading rate (OLR) of 1.5 g chemical oxygen demand (COD)/l-d. After 102 days, 2AB was not degraded at all and the reactor operation was shifted to batch mode by recycling the effluent. After some days, 2AB was completely mineralized and accumulation of flocculent sludge was observed. Batch biodegradability assays demonstrated that this flocculent biomass had a higher specific biodegradation rate compared to the granular sludge. During EGSB reactor operation, the flocculent biomass which was not attached to the granules probably washed-out under the applied hydrodynamic conditions. When the EGSB reactor was operated at V(up) of 2 m/h to favour the retention of the flocculent biomass, 2AB was mineralized even at influent concentrations as low as 140 mg COD/L. Kinetic calculations indicated that the sludge had an apparent K(s) value for the mineralization of 2AB as low as 24 mg COD/l.
KW - Anaerobic degradation
KW - Anthranilic acid
KW - Aromatic amines
KW - EGSB reactor
KW - Flocculent biomass
KW - Granular sludge
KW - Methanogenic conditions
UR - http://www.scopus.com/inward/record.url?scp=0032702367&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032702367&partnerID=8YFLogxK
U2 - 10.1016/S0273-1223(99)00625-3
DO - 10.1016/S0273-1223(99)00625-3
M3 - Article
AN - SCOPUS:0032702367
VL - 40
SP - 187
EP - 194
JO - Water Science and Technology
JF - Water Science and Technology
SN - 0273-1223
IS - 8
ER -