TRH1 encodes a potassium transporter required for tip growth in arabidopsis root hairs

S. Rigas, G. Debrosses, K. Haralampidis, F. Vicente-Agullo, Kenneth A Feldmann, A. Grabov, L. Dolan, P. Hatzopoulos

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

Root hair initiation involves the formation of a bulge at the basal end of the trichoblast by localized diffuse growth. Tip growth occurs subsequently at this initiation site and is accompanied by the establishment of a polarized cytoplasmic organization. Arabidopsis plants homozygous for a complete loss-of-function tiny root hair 1 (trh1) mutation were generated by means of the T-DNA-tagging method. Trichoblasts of trh1 plants form initiation sites but fail to undergo tip growth. A predicted primary structure of TRH1 indicates that it belongs to the AtKT/AtKUP/HAK K+ transporter family. The proposed function of TRH1 as a K+ transporter was confirmed in 86Rb uptake experiments, which demonstrated that trh1 plants are partially impaired in K+ transport. In line with these results, TRH1 was able to complement the trk1 potassium transporter mutant of Saccharomyces, which is defective in high-affinity K+ uptake. Surprisingly, the trh1 phenotype was not restored when mutant seedlings were grown at high external potassium concentrations. These data demonstrate that TRH1 mediates K+ transport in Arabidopsis roots and is responsible for specific K+ translocation, which is essential for root hair elongation.

Original languageEnglish (US)
Pages (from-to)139-151
Number of pages13
JournalPlant Cell
Volume13
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

root hairs
Arabidopsis
transporters
Potassium
potassium
Growth
Saccharomyces
Seedlings
Elongation
Phenotype
uptake mechanisms
mutants
Mutation
plant architecture
complement
Experiments
mutation
phenotype
seedlings
DNA

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Rigas, S., Debrosses, G., Haralampidis, K., Vicente-Agullo, F., Feldmann, K. A., Grabov, A., ... Hatzopoulos, P. (2001). TRH1 encodes a potassium transporter required for tip growth in arabidopsis root hairs. Plant Cell, 13(1), 139-151. https://doi.org/10.1105/tpc.13.1.139

TRH1 encodes a potassium transporter required for tip growth in arabidopsis root hairs. / Rigas, S.; Debrosses, G.; Haralampidis, K.; Vicente-Agullo, F.; Feldmann, Kenneth A; Grabov, A.; Dolan, L.; Hatzopoulos, P.

In: Plant Cell, Vol. 13, No. 1, 2001, p. 139-151.

Research output: Contribution to journalArticle

Rigas, S, Debrosses, G, Haralampidis, K, Vicente-Agullo, F, Feldmann, KA, Grabov, A, Dolan, L & Hatzopoulos, P 2001, 'TRH1 encodes a potassium transporter required for tip growth in arabidopsis root hairs', Plant Cell, vol. 13, no. 1, pp. 139-151. https://doi.org/10.1105/tpc.13.1.139
Rigas, S. ; Debrosses, G. ; Haralampidis, K. ; Vicente-Agullo, F. ; Feldmann, Kenneth A ; Grabov, A. ; Dolan, L. ; Hatzopoulos, P. / TRH1 encodes a potassium transporter required for tip growth in arabidopsis root hairs. In: Plant Cell. 2001 ; Vol. 13, No. 1. pp. 139-151.
@article{4fe35b12e8ac460b890a3e46744200c9,
title = "TRH1 encodes a potassium transporter required for tip growth in arabidopsis root hairs",
abstract = "Root hair initiation involves the formation of a bulge at the basal end of the trichoblast by localized diffuse growth. Tip growth occurs subsequently at this initiation site and is accompanied by the establishment of a polarized cytoplasmic organization. Arabidopsis plants homozygous for a complete loss-of-function tiny root hair 1 (trh1) mutation were generated by means of the T-DNA-tagging method. Trichoblasts of trh1 plants form initiation sites but fail to undergo tip growth. A predicted primary structure of TRH1 indicates that it belongs to the AtKT/AtKUP/HAK K+ transporter family. The proposed function of TRH1 as a K+ transporter was confirmed in 86Rb uptake experiments, which demonstrated that trh1 plants are partially impaired in K+ transport. In line with these results, TRH1 was able to complement the trk1 potassium transporter mutant of Saccharomyces, which is defective in high-affinity K+ uptake. Surprisingly, the trh1 phenotype was not restored when mutant seedlings were grown at high external potassium concentrations. These data demonstrate that TRH1 mediates K+ transport in Arabidopsis roots and is responsible for specific K+ translocation, which is essential for root hair elongation.",
author = "S. Rigas and G. Debrosses and K. Haralampidis and F. Vicente-Agullo and Feldmann, {Kenneth A} and A. Grabov and L. Dolan and P. Hatzopoulos",
year = "2001",
doi = "10.1105/tpc.13.1.139",
language = "English (US)",
volume = "13",
pages = "139--151",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "1",

}

TY - JOUR

T1 - TRH1 encodes a potassium transporter required for tip growth in arabidopsis root hairs

AU - Rigas, S.

AU - Debrosses, G.

AU - Haralampidis, K.

AU - Vicente-Agullo, F.

AU - Feldmann, Kenneth A

AU - Grabov, A.

AU - Dolan, L.

AU - Hatzopoulos, P.

PY - 2001

Y1 - 2001

N2 - Root hair initiation involves the formation of a bulge at the basal end of the trichoblast by localized diffuse growth. Tip growth occurs subsequently at this initiation site and is accompanied by the establishment of a polarized cytoplasmic organization. Arabidopsis plants homozygous for a complete loss-of-function tiny root hair 1 (trh1) mutation were generated by means of the T-DNA-tagging method. Trichoblasts of trh1 plants form initiation sites but fail to undergo tip growth. A predicted primary structure of TRH1 indicates that it belongs to the AtKT/AtKUP/HAK K+ transporter family. The proposed function of TRH1 as a K+ transporter was confirmed in 86Rb uptake experiments, which demonstrated that trh1 plants are partially impaired in K+ transport. In line with these results, TRH1 was able to complement the trk1 potassium transporter mutant of Saccharomyces, which is defective in high-affinity K+ uptake. Surprisingly, the trh1 phenotype was not restored when mutant seedlings were grown at high external potassium concentrations. These data demonstrate that TRH1 mediates K+ transport in Arabidopsis roots and is responsible for specific K+ translocation, which is essential for root hair elongation.

AB - Root hair initiation involves the formation of a bulge at the basal end of the trichoblast by localized diffuse growth. Tip growth occurs subsequently at this initiation site and is accompanied by the establishment of a polarized cytoplasmic organization. Arabidopsis plants homozygous for a complete loss-of-function tiny root hair 1 (trh1) mutation were generated by means of the T-DNA-tagging method. Trichoblasts of trh1 plants form initiation sites but fail to undergo tip growth. A predicted primary structure of TRH1 indicates that it belongs to the AtKT/AtKUP/HAK K+ transporter family. The proposed function of TRH1 as a K+ transporter was confirmed in 86Rb uptake experiments, which demonstrated that trh1 plants are partially impaired in K+ transport. In line with these results, TRH1 was able to complement the trk1 potassium transporter mutant of Saccharomyces, which is defective in high-affinity K+ uptake. Surprisingly, the trh1 phenotype was not restored when mutant seedlings were grown at high external potassium concentrations. These data demonstrate that TRH1 mediates K+ transport in Arabidopsis roots and is responsible for specific K+ translocation, which is essential for root hair elongation.

UR - http://www.scopus.com/inward/record.url?scp=0035105236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035105236&partnerID=8YFLogxK

U2 - 10.1105/tpc.13.1.139

DO - 10.1105/tpc.13.1.139

M3 - Article

VL - 13

SP - 139

EP - 151

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 1

ER -